Pancreas Research Group and Division of Gut Microbiome Research, Wellcome DBT India Alliance Laboratories, Institute of Basic and Translational Research, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, Telangana, 500032, India.
Department of Medical Gastroenterology, Asian Institute of Gastroenterology, Hyderabad, India.
Sci Rep. 2021 May 26;11(1):10978. doi: 10.1038/s41598-021-90024-w.
We hypothesized that the gut microbiome in patients with diabetes secondary to chronic pancreatitis (Type 3c) is different from those with Type 1 and Type 2 diabetes. This was a cross-sectional preliminary study that included 8 patients with Type 1, 10 with Type 2, 17 with Type 3c diabetes and 9 healthy controls. Demographic, clinical, biochemical, imaging and treatment data were recorded and sequencing of the V3-V4 region of the bacterial 16SrRNA was done on fecal samples. Bioinformatics and statistical analyses was performed to evaluate the differences in the diversity indices, distance matrices, relative abundances and uniqueness of organisms between the types of diabetes. There was significant difference in the species richness. Beta diversity was significantly different between patients with Type 3c diabetes and the other groups. 31 genera were common to all the three types of diabetes. There was significant differences in the species level taxa between Type 3c diabetes and the other groups. The unique bacterial species signature in Type 3c diabetes compared to Type 1 and Type 2 diabetes included Nesterenkonia sp. AN1, Clostridium magnum, Acinetobacter lwoffii, Clostridium septicum, Porphyromonas somerae, Terrabacter tumescens, and Synechococus sp.
我们假设继发于慢性胰腺炎的糖尿病（3c 型）患者的肠道微生物群与 1 型和 2 型糖尿病患者不同。这是一项横断面初步研究，纳入了 8 例 1 型糖尿病患者、10 例 2 型糖尿病患者、17 例 3c 型糖尿病患者和 9 例健康对照者。记录了人口统计学、临床、生化、影像学和治疗数据，并对粪便样本进行细菌 16SrRNA V3-V4 区的测序。进行生物信息学和统计学分析，以评估不同类型糖尿病之间多样性指数、距离矩阵、相对丰度和生物体独特性的差异。在物种丰富度方面存在显著差异。3c 型糖尿病患者与其他组之间的β多样性存在显著差异。31 个属在所有 3 种类型的糖尿病中都很常见。3c 型糖尿病与其他组之间在种水平分类群方面存在显著差异。与 1 型和 2 型糖尿病相比，3c 型糖尿病特有的细菌种属包括 Nesterenkonia sp. AN1、Clostridium magnum、Acinetobacter lwoffii、Clostridium septicum、Porphyromonas somerae、Terrabacter tumescens 和 Synechococus sp.
