Inabu Yudai, Yamamoto Hiroshi, Yamano Haruki, Taguchi Yutaka, Okada Shunnosuke, Etoh Tetsuji, Shiotsuka Yuji, Fujino Ryoichi, Takahashi Hideyuki
Kuju Agricultural Research Center, Graduate School of Agriculture, Kyushu University, Oita, 878-0201, Japan.
Heliyon. 2021 May 15;7(5):e07046. doi: 10.1016/j.heliyon.2021.e07046. eCollection 2021 May.
Bovine colostrum contains growth factors, cytokines, hormones, and enzymes, which have important roles in stimulating gastrointestinal development of neonatal calves. In the present study, we measured the concentration of glucagon-like peptide 2 (GLP-2), one of the gut-derived peptides secreted from intestinal L-cells, in colostrum and transition milk of Japanese black cattle. All colostrum samples were collected within 24 h after calving (d 0) and transition milk was collected at 24, 48 and 72 h relative to the time at colostrum sampling (d 1, d 2 and d 3, respectively). Concentrations of GLP-2 in colostrum were 5.53 ± 1.07 ng/mL on average (range = 0.94-9.60 ng/mL) and decreased from d 0 to 3 ( < 0.01). Furthermore, concentrations of GLP-2 in colostrum and transition milk were quadratically decreased with the elapsed time from parturition until colostrum sampling (R = 0.48, < 0.01). Our results show for the first time that GLP-2 is present in bovine colostrum and transition milk and that concentrations decreased with elapsed time from parturition.
牛初乳含有生长因子、细胞因子、激素和酶，这些物质在刺激新生犊牛胃肠道发育中发挥着重要作用。在本研究中，我们测定了日本黑牛初乳和过渡乳中胰高血糖素样肽2（GLP-2）的浓度，GLP-2是一种由肠道L细胞分泌的肠道衍生肽。所有初乳样本均在产犊后24小时内（第0天）采集，过渡乳在相对于初乳采样时间的24、48和72小时采集（分别为第1、2和3天）。初乳中GLP-2的平均浓度为5.53±1.07 ng/mL（范围 = 0.94 - 9.60 ng/mL），从第0天到第3天浓度下降（P<0.01）。此外，初乳和过渡乳中GLP-2的浓度随着从分娩到初乳采样的时间延长呈二次下降（R = 0.48，P<0.01）。我们的结果首次表明GLP-2存在于牛初乳和过渡乳中，并且其浓度随着分娩后时间的推移而降低。
