Department of Plastic and Reconstructive Surgery, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Department of Biotechnology Engineering, Avram and Stella Goldstein-Goren, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Aesthetic Plast Surg. 2021 Dec;45(6):2980-2989. doi: 10.1007/s00266-021-02348-7. Epub 2021 May 26.
Post-operative infection is a significant complication of breast implant surgery that may require extensive use of antibiotics and surgical interventions. Here, we developed a biomaterial coating that is chemically bonded to silicone implants which delivers antimicrobial ions over time.
After coating the silicone implants with a "mediator" polymer (γ-PGA), the implants were impregnated with silver (Ag) ions. Antimicrobial effects of these implants were assayed with modified Kirby-Bauer disk diffusion method. The silicone disks were transferred to a plate with fresh bacteria. Control was intended to simulate an intra-operative wash.
The Ag-γ-PGA coated silicone demonstrated antimicrobial effects against the most common etiological agents of breast implant infections, including Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Klebsiella pneumoniae. There was no effect of inhibition of bacterial growth around the control silicone or the silicone coated only with γ-PGA. The zone of inhibition was generally larger around the Ag-γ-PGA coated silicone as compared to the silicone irrigated with gentamicin, and continued antibacterial effect was also observed at 48 hours in the Ag-γ-PGA coated silicone for all bacteria groups with the exception of P. aeruginosa. Gentamicin-irrigated silicone did not inhibit bacterial growth at 48 hours.
The observed antibacterial performance of the Ag-γ-PGA coating as compared to simulated intra-operative antibiotic wash is promising and should be further evaluated to develop the next generation of implants with diminished risk for post-operative implant infections.
术后感染是乳房植入物手术的一个重大并发症，可能需要广泛使用抗生素和手术干预。在这里，我们开发了一种生物材料涂层，它可以与硅树脂植入物化学结合，随着时间的推移释放抗菌离子。
在硅树脂植入物上涂覆“介质”聚合物（γ-PGA）后，将植入物用银（Ag）离子浸渍。用改良 Kirby-Bauer 圆盘扩散法测定这些植入物的抗菌效果。将硅树脂圆盘转移到一个装有新鲜细菌的平板上。对照旨在模拟术中冲洗。
Ag-γ-PGA 涂层硅树脂对乳房植入物感染最常见的病原体具有抗菌作用，包括铜绿假单胞菌、金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌和肺炎克雷伯菌。控制组对抑制细菌生长没有影响，对照组只有 γ-PGA 涂层的硅树脂也是如此。与用庆大霉素冲洗的硅树脂相比，Ag-γ-PGA 涂层硅树脂周围的抑菌区通常更大，除铜绿假单胞菌外，所有细菌组在 Ag-γ-PGA 涂层硅树脂中观察到的持续抗菌作用也持续到 48 小时。庆大霉素冲洗的硅树脂在 48 小时内不能抑制细菌生长。
与模拟术中抗生素冲洗相比，Ag-γ-PGA 涂层的抗菌性能有希望，应进一步评估，以开发出新一代植入物，降低术后植入物感染的风险。
