Zaręba-Marchewka Kinga, Bomba Arkadiusz, Scharf Sabine, Niemczuk Krzysztof, Schnee Christiane, Szymańska-Czerwińska Monika
Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
Department of Omics Analyses, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
Pathogens. 2023 Jun 29;12(7):891. doi: 10.3390/pathogens12070891.
is an intracellular bacterium belonging to the family. Poultry is considered to be the major reservoir of this agent, which has worldwide distribution and a particularly consistent worldwide occurrence in chicken flocks. The bacterium has been linked to respiratory disease in humans but without definitive confirmation; nevertheless, while it has not been proved to be the cause of human respiratory disease, a recent report from Italy verified its bird-to-human transmission. This aspect being significant for public health, more research is needed to gain insight into the infection biology of . In this study, the genomes of eleven novel field strains from different regions of Poland were analyzed comparatively. It was confirmed that strains are closely related, with at least 99.46% sequence identity. They possess a conservative genome structure involving the plasticity zone with a complete cytotoxin, the type three secretion system, inclusion membrane proteins, polymorphic membrane proteins, A and B histone-like proteins, and the chlamydial protease-like activating factor exoenzyme, as well as plasmids. Genetic diversity seems to be restricted. However, some genetic loci, such as A and multi-locus sequence typing target genes, are diverse enough to enable high-resolution genotyping and epidemiological tracing.
是一种属于该科的细胞内细菌。家禽被认为是这种病原体的主要储存宿主，它在全球范围内分布，在鸡群中全球发生率特别一致。这种细菌与人类呼吸道疾病有关，但未经明确证实；然而，虽然尚未证明它是人类呼吸道疾病的病因，但意大利最近的一份报告证实了其从禽类到人类的传播。这一方面对公共卫生意义重大，需要更多研究来深入了解该菌的感染生物学。在本研究中，对来自波兰不同地区的11株新型该菌田间菌株的基因组进行了比较分析。证实这些菌株密切相关，序列同一性至少为99.46%。它们具有保守的基因组结构，包括带有完整细胞毒素的可塑性区、三型分泌系统、包涵体膜蛋白、多态性膜蛋白、A和B组蛋白样蛋白以及衣原体蛋白酶样激活因子外切酶，还有质粒。遗传多样性似乎受到限制。然而，一些基因位点，如A和多位点序列分型靶基因，具有足够的多样性以实现高分辨率基因分型和流行病学追踪。
