Microbiology Department, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Department of Medical Laboratory Sciences, Khomein University of Medical Sciences, Khomein, Iran.
Ann Clin Microbiol Antimicrob. 2021 May 27;20(1):40. doi: 10.1186/s12941-021-00442-x.
Treatment of burn wound infections has become a global challenge due to the spread of multidrug-resistant bacteria; therefore, the development of new treatment options for the mentioned infections is essential. Platelets have drawn much attention for this purpose because they are a safe and cost-effective source of different antimicrobial peptides and growth factors. The present study evaluated antibacterial effects and wound healing properties of Platelet-derived Biomaterial (PdB) against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections.
PdB was prepared through the freezing and thawing process and then, in vitro antibacterial effect was determined by disk diffusion and broth microdilution methods. Afterward, burn wound was inflicted on 56 rats, infected with both bacteria, and topical administration was performed to evaluate antibacterial effects and wound healing properties of PdB.
In vitro results showed that PdB inhibited the growth of A. baumannii in the highest dose (0.5), while we did not detect any inhibitory effects against K. pneumoniae. By contrast, PdB significantly inhibited the growth of bacteria in treated animal wounds compared to the control groups (P value < 0.05). Macroscopic assessments pointed to the significant enhancement of wound closure in the treated animals. In addition, histopathological examination demonstrated that treatment of rats with PdB led to a considerable increase in re-epithelialization and attenuated the formation of granulation tissue (P value < 0.05).
The use of topical PdB is an attractive strategy for treating A. baumannii and K. pneumoniae burn wound infections because it inhibits bacterial growth and promotes wound healing properties.
由于多药耐药菌的传播，烧伤创面感染的治疗已成为全球性挑战；因此，开发针对上述感染的新治疗方案至关重要。血小板因其是不同抗菌肽和生长因子的安全且具有成本效益的来源而备受关注。本研究评估了血小板衍生生物材料（PdB）对鲍曼不动杆菌和肺炎克雷伯菌烧伤创面感染的抗菌作用和促愈合特性。
通过冻融过程制备 PdB，然后通过圆盘扩散和肉汤微量稀释法测定其体外抗菌效果。随后，在 56 只大鼠上造成烧伤创面感染上述两种细菌，并进行局部给药，以评估 PdB 的抗菌作用和促愈合特性。
体外结果表明，PdB 在最高剂量（0.5）下抑制了鲍曼不动杆菌的生长，而对肺炎克雷伯菌没有检测到任何抑制作用。相比之下，与对照组相比，PdB 显著抑制了治疗动物创面中细菌的生长（P 值<0.05）。宏观评估表明，治疗动物的创面闭合明显得到了改善。此外，组织病理学检查表明，用 PdB 治疗大鼠可显著增加上皮再形成，并减轻肉芽组织的形成（P 值<0.05）。
局部应用 PdB 是治疗鲍曼不动杆菌和肺炎克雷伯菌烧伤创面感染的一种有吸引力的策略，因为它能抑制细菌生长并促进创面愈合特性。
