College of Chemistry, Jilin University, Changchun 130012, PR China.
College of Resources and Environment, Jilin Agricultural University, Changchun 130118, PR China.
Carbohydr Polym. 2021 Aug 15;266:118174. doi: 10.1016/j.carbpol.2021.118174. Epub 2021 May 7.
In this study, a biocompatible folate-decorated reductive-responsive carboxymethylcellulose-based nanocapsules (FA-RCNCs) were designed and prepared via sonochemical method for targeted delivery and controlled release of hydrophobic drugs. The shell of FA-RCNCs was cross-linked by disulfide bonds formed from hydrosulfuryl groups on the thiolated carboxymethylcellulose (TCMC) and encapsulated hydrophobic drug dispersed in the oil phase into nanocapsules. Moreover, the size and morphology of drug loaded FA-RCNCs were characterized by DLS, SEM and CLSM which indicated that the synthesized nanocapsules have suitable size range and excellent stability for circulating in the bloodstream. The drug release rate of FA-RCNCs could be controlled by adjusting their sizes and shell thickness, which could be dominated by the concentration of TCMC and sonochemical conditions. Furthermore, the obtained FA-RCNCs could be ingested into Hela cells via folate-receptor (FR)-mediated endocytosis and quickly release drugs under reductive environment, which demonstrated that FA-RCNCs could become potential hydrophobic drugs carries for cancer therapy.
在这项研究中，通过超声化学方法设计并制备了一种具有生物相容性的叶酸修饰的还原响应性羧甲基纤维素纳米胶囊（FA-RCNCs），用于疏水性药物的靶向传递和控制释放。FA-RCNCs 的壳由巯基化羧甲基纤维素（TCMC）上的氢硫基形成的二硫键交联，并将疏水性药物包封在油相中分散在纳米胶囊内。此外，通过 DLS、SEM 和 CLSM 对载药 FA-RCNCs 的粒径和形态进行了表征，结果表明合成的纳米胶囊具有合适的粒径范围和优异的血液循环稳定性。通过调整其粒径和壳层厚度可以控制 FA-RCNCs 的药物释放速率，这可以通过 TCMC 的浓度和超声化学条件来控制。此外，所得的 FA-RCNCs 可以通过叶酸受体（FR）介导的内吞作用被 Hela 细胞摄取，并在还原环境下快速释放药物，这表明 FA-RCNCs 可以成为用于癌症治疗的潜在疏水性药物载体。
