miR-4535和miR-191-5p作为严重绒毛膜羊膜炎独立生物标志物在母血清中的临床意义
Clinical Significance of miR-4535 and miR-191-5p in Maternal Serum as Independent Biomarkers for Severe Chorioamnionitis.
作者信息
Ishida Koko, Kiyoshima Chihiro, Urushiyama Daichi, Hirakawa Toyofumi, Imi Shiori, Hamasaki Makoto, Nagamitsu Shinichiro, Nomiyama Makoto, Hata Kenichiro, Yotsumoto Fusanori
机构信息
Department of Obstetrics and Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, JPN.
Department of Pathology, Faculty of Medicine, Fukuoka University, Fukuoka, JPN.
出版信息
Cureus. 2024 Oct 22;16(10):e72120. doi: 10.7759/cureus.72120. eCollection 2024 Oct.
INTRODUCTION
Chorioamnionitis, a perinatal condition caused by fetal membrane inflammation, results in preterm birth, neonatal sepsis, necrotizing enterocolitis, and brain disease in infants. However, predicting maternal and fetal prognoses is challenging. We aimed to assess the relationship between fetal infection induced by severe chorioamnionitis or morbidity and the expression levels of serum miR-4535, miR-1915-5p, and miR-191-5p levels, which are promising biomarkers for chorioamnionitis, in pregnant women with chorioamnionitis.
METHODS
We collectedserum and amniotic fluid samples from 40 pregnant women with preterm labor and analyzed miR-4535, miR-1915-5p, and miR-191-5p expressions. We calculated the area under the curve (AUC) and Youden index to examine the diagnostic accuracy of infection-induced fetal morbidity.
RESULTS
The serum miR-4535 and miR-191-5p levels were significantly higher in patients with severe chorioamnionitis than in those with chorionitis or sub-chorionitis ( = 0.001 and 0.003, respectively). The AUC of miR-4535 and miR-191-5p (0.864 and 0.836, respectively) indicated their good diagnostic accuracy for severe chorioamnionitis. Significant correlations were observed between serum and amniotic fluid miR-4535 expression ( = 0.011) and serum miR-4535 and miR-191-5p expressions. miR-4535 AUC accurately predicted elevated neonatal immunoglobulin M level (AUC = 0.922) and infection-induced fetal morbidity (AUC = 0.805).
CONCLUSION
Serum miR-4535 and miR-191-5p are associated with infection-induced severe chorioamnionitis and fetal morbidity and maternal infection, respectively.
引言
绒毛膜羊膜炎是一种由胎膜炎症引起的围产期疾病，可导致早产、新生儿败血症、坏死性小肠结肠炎和婴儿脑部疾病。然而，预测母婴预后具有挑战性。我们旨在评估患有绒毛膜羊膜炎的孕妇中，由严重绒毛膜羊膜炎或发病引起的胎儿感染与血清miR-4535、miR-1915-5p和miR-191-5p水平的表达之间的关系，这些都是绒毛膜羊膜炎很有前景的生物标志物。
方法
我们收集了40例早产孕妇的血清和羊水样本，并分析了miR-4535、miR-1915-5p和miR-191-5p的表达。我们计算曲线下面积（AUC）和尤登指数，以检验感染引起的胎儿发病的诊断准确性。
结果
严重绒毛膜羊膜炎患者的血清miR-4535和miR-191-5p水平显著高于绒毛膜炎或绒毛膜下炎患者（分别为P = 0.001和0.003）。miR-4535和miR-191-5p的AUC（分别为0.864和0.836）表明它们对严重绒毛膜羊膜炎具有良好的诊断准确性。血清和羊水miR-4535表达之间（P = 0.011）以及血清miR-4535和miR-191-5p表达之间存在显著相关性。miR-4535的AUC准确预测了新生儿免疫球蛋白M水平升高（AUC = 0.922）和感染引起的胎儿发病（AUC = 0.805）。
结论
血清miR-4535和miR-191-5p分别与感染引起的严重绒毛膜羊膜炎、胎儿发病和母体感染相关。
Zotero 插件