长链非编码RNA RUNDC3A-AS1通过靶向miR-182-5p/ADAM9促进甲状腺癌肺转移。
Long Non-coding RNA RUNDC3A-AS1 Promotes Lung Metastasis of Thyroid Cancer via Targeting the miR-182-5p/ADAM9.
作者信息
Ma Dawei, Zhu Yan, Zhang Xiao, Zhang Jia, Chen Wei, Chen Xinyuan, Qian Yichun, Zhao Yanbin, Hu Tingting, Yao Zhangyu, Zhao Wei, Zhang Yuan, Liu Fangzhou
机构信息
Department of Pathology, The Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing, China.
Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
出版信息
Front Cell Dev Biol. 2021 May 11;9:650004. doi: 10.3389/fcell.2021.650004. eCollection 2021.
Long non-coding RNAs (lncRNAs) have been identified as influential indicators in variety of malignancies. Among which, LncRNA RUNDC3A-AS1 is reported to upregulate in thyroid cancer. However, the expression pattern and the pathological function of lncRNA RUNDC3A-AS1 in thyroid cancer is unclear. In this study, we examined the expression levels of lncRNA RUNDC3A-AS1 in the thyroid cancer tissues and cell lines via RT-qPCR analysis. The effects of RUNDC3A-AS1 on thyroid cancer cell metastasis were detected by transwell chamber assay, scratch assay and lung metastasis model . The results indicated that RUNDC3A-AS1 was highly expressed in the thyroid cancer tissues and cell lines. Functionally, knockdown of RUNDC3A-AS1 could repress the migration and invasion of thyroid cancer cells , and inhibit thyroid cancer metastasis to lung . Mechanistically, RUNDC3A-AS1 served as an inhibitor of miR-182-5p in tumor tissues and cell lines. RUNDC3A-AS1 inhibited the expression of miR-182-5p to increase the expression level of ADAM9, thus further aggravating the malignancy of thyroid cancer. Therefore, the RUNDC3A-AS1/miR-182-5p/ADAM9 axis may be a potential therapeutic target for the treatment of thyroid cancer metastasis.
长链非编码RNA（lncRNAs）已被确定为多种恶性肿瘤中有影响力的指标。其中，据报道lncRNA RUNDC3A-AS1在甲状腺癌中上调。然而，lncRNA RUNDC3A-AS1在甲状腺癌中的表达模式和病理功能尚不清楚。在本研究中，我们通过RT-qPCR分析检测了lncRNA RUNDC3A-AS1在甲状腺癌组织和细胞系中的表达水平。通过Transwell小室实验、划痕实验和肺转移模型检测RUNDC3A-AS1对甲状腺癌细胞转移的影响。结果表明，RUNDC3A-AS1在甲状腺癌组织和细胞系中高表达。在功能上，敲低RUNDC3A-AS1可抑制甲状腺癌细胞的迁移和侵袭，并抑制甲状腺癌向肺转移。机制上，RUNDC3A-AS1在肿瘤组织和细胞系中作为miR-182-5p的抑制剂。RUNDC3A-AS1抑制miR-182-5p的表达以增加ADAM9的表达水平，从而进一步加剧甲状腺癌的恶性程度。因此，RUNDC3A-AS1/miR-182-5p/ADAM9轴可能是治疗甲状腺癌转移的潜在治疗靶点。
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