Department of Life Sciences, University of Tokyo, Tokyo, Japan.
Tohoku University, Sendai, Miyagi, Japan.
Methods Mol Biol. 2021;2295:455-468. doi: 10.1007/978-1-0716-1362-7_26.
Pathways of lipid biosynthesis are highly complex and have been established in model organisms such as Arabidopsis thaliana and Chlamydomonas reinhardtii, whereas in other organisms, we need bioinformatic tools to map individual enzymes onto reference pathways. In this chapter, we explain representative tools that are useful in identifying algal orthologs of lipid biosynthetic enzymes and finding new enzymes that are possibly involved in the pathway of interest. All descriptions in this chapter refer to in silico (i.e., computer-based) methods rather than laboratory experiments.
脂质生物合成途径非常复杂,在拟南芥和莱茵衣藻等模式生物中已得到确立,而在其他生物中,我们需要生物信息学工具将单个酶映射到参考途径上。在本章中,我们将解释在鉴定脂质生物合成酶的藻类同源物和发现可能参与感兴趣途径的新酶方面有用的代表性工具。本章中的所有描述均指基于计算机的(即计算机)方法,而不是实验室实验。