近红外荧光镁探针的研制及其在细胞内信号多色成像中的应用。
Development of Near-Infrared Fluorescent Mg Probe and Application to Multicolor Imaging of Intracellular Signals.
机构信息
Department of Biosciences and Informatics, Keio University, Yokohama, Kanagawa, Japan.
Department of Applied Chemistry, Keio University, Yokohama, Kanagawa, Japan.
出版信息
Methods Mol Biol. 2021;2274:217-235. doi: 10.1007/978-1-0716-1258-3_19.
Recent extensive studies revealed that the intracellular concentration of magnesium ions (Mg) is one of the important factors to regulate cellular functions. To evaluate the impact of Mg concentration changes on intracellular signals or events, simultaneous imaging of Mg with those phenomena is a powerful technique. The present protocol describes the synthesis and evaluation of near-infrared (NIR) fluorescent Mg-selective probes, named KMG-500 series, and the application to simultaneous imaging of the corresponding intracellular signal transductions and molecular events. The present protocol for multicolor imaging using fluorescent probes in the NIR and visible ranges is highly useful to reveal how multiple molecular events are correlated each other in each single cell.
最近的广泛研究表明，镁离子（Mg）的细胞内浓度是调节细胞功能的重要因素之一。为了评估 Mg 浓度变化对细胞内信号或事件的影响，同时对 Mg 与这些现象进行成像，这是一种强大的技术。本方案描述了近红外（NIR）荧光 Mg 选择性探针，命名为 KMG-500 系列的合成和评价，以及在相应的细胞内信号转导和分子事件的同时成像中的应用。本方案使用近红外和可见范围内的荧光探针进行多色成像，对于揭示每个单个细胞中多个分子事件如何相互关联非常有用。
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