The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Ibaraki, Japan.
Methods Mol Biol. 2021;2274:295-304. doi: 10.1007/978-1-0716-1258-3_25.
The application of smartphones as detectors is essential to achieve ubiquitous measurement targeting biomolecules. Because bioluminescence (BL), as a tag for a target sample, does not require an excitation light source, it can be combined with a smartphone to constitute a compact and mobile measurement system. A method was recently established to detect the spectral change of ratiometric indicators based on bioluminescence resonance energy transfer with a smartphone camera. For example, it was possible to detect changes in the BL color of the Ca indicator quantitatively and easily calculate the concentration of free Ca by setting appropriate image acquisition conditions in a smartphone application. In this paper, we describe techniques to obtain scientifically relevant and reliable BL data with such a convenient instrument. This protocol expands the potential of the smartphone as a personal imaging device with high mobility that can be used anywhere.
智能手机作为探测器的应用对于实现针对生物分子的普遍测量至关重要。因为生物发光（BL）作为目标样本的标记物，不需要激发光源，所以可以与智能手机结合构成紧凑、移动的测量系统。最近，人们建立了一种方法，利用智能手机摄像头检测基于生物发光共振能量转移的比率指示剂的光谱变化。例如，可以通过在智能手机应用程序中设置适当的图像采集条件，定量检测 Ca 指示剂的 BL 颜色变化，并轻松计算游离 Ca 的浓度。在本文中，我们描述了使用这种便捷仪器获取具有科学相关性和可靠性的 BL 数据的技术。该方案扩展了智能手机作为可在任何地方使用的高移动性个人成像设备的潜力。
