SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka, Japan.
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
Methods Mol Biol. 2022;2525:219-226. doi: 10.1007/978-1-0716-2473-9_16.
Bioluminescent indicators facilitate determination of bioactive molecules in blood samples with high sensitivity. Using a bright luciferase, its bioluminescence (BL) can be easily detected by conventional light sensing devices. In this chapter, we describe a protocol to measure bioactive molecules in blood by taking the BL images with a smartphone camera. We exemplify the measurement of unconjugated bilirubin (UCBR) concentration in the blood of mice using a ratiometric bioluminescent UCBR indicator, BABI (bilirubin assessment with a bioluminescent indicator), and a smartphone camera. We show the UCBR concentration is easily determined through measuring the variance in the BL color with a smartphone camera. This method provides a practical method to lead to future point-of-care diagnosis with quick and simple procedures.
生物发光指示剂有助于高灵敏度地确定血液样本中的生物活性分子。使用明亮的荧光素酶，其生物发光 (BL) 可以很容易地被传统的光感测设备检测到。在本章中，我们描述了一种使用智能手机摄像头拍摄 BL 图像来测量血液中生物活性分子的方案。我们使用比率型生物发光 UCBR 指示剂 BABI（胆红素的生物发光指示剂评估）和智能手机摄像头来例证测量小鼠血液中非结合胆红素 (UCBR) 浓度的方法。我们展示了通过使用智能手机摄像头测量 BL 颜色的变化，很容易确定 UCBR 浓度。这种方法为未来的即时诊断提供了一种实用的方法，具有快速简单的操作流程。
