Hu Yuan, Rubin Julia, Mussio Kaitlyn, Riley Lee W
Division of Epidemiology, School of Public Health, University of California Berkeley, Berkeley, CA 94720, USA.
Division of Environmental Health Science, School of Public Health, University of California Berkeley, Berkeley, CA 94720, USA.
J Glob Antimicrob Resist. 2021 Sep;26:166-173. doi: 10.1016/j.jgar.2021.05.004. Epub 2021 May 26.
Bacterial antimicrobial resistance is a serious global public-health threat. Intestinal commensal drug-resistant bacteria have been suggested as an important reservoir of antimicrobial resistance genes (ARGs), which may be acquired via food. We aimed to identify risk factors associated with faecal carriage of drug-resistant commensal Escherichia coli among healthy adults focused on their dietary habits.
We conducted a cross-sectional study targeting healthy adult volunteers in a college community. Faecal samples and questionnaires were obtained from 113 volunteers. We conducted backward elimination logistic regression and least absolute shrinkage and selection operator (LASSO) methods to identify risk factors.
We analysed responses from 81 of 113 volunteers who completed the questionnaire. The logistic regression and LASSO methods identified red meat consumption to be associated with an increased risk [OR = 6.13 (95% CI 1.83-24.2) and 1.82, respectively] and fish consumption with a reduced risk [OR = 0.27 (95% CI 0.08-0.85) and 0.81] for carriage of multidrug-resistant (MDR) E. coli, adjusted for biological sex, employment status, frequently used supermarket and previous travel.
Dietary habits are associated with risk of faecal carriage of MDR E. coli. This study supports the growing evidence that food may be an important source of ARGs present in human commensal E. coli.
细菌对抗菌药物的耐药性是全球严重的公共卫生威胁。肠道共生耐药菌被认为是抗菌药物耐药基因（ARGs）的重要储存库，这些基因可能通过食物获得。我们旨在确定健康成年人中与耐药共生大肠杆菌粪便携带相关的危险因素，重点关注他们的饮食习惯。
我们在一个大学社区针对健康成年志愿者进行了一项横断面研究。从113名志愿者那里获取了粪便样本和问卷。我们采用向后逐步消除逻辑回归和最小绝对收缩和选择算子（LASSO）方法来确定危险因素。
我们分析了113名完成问卷的志愿者中81人的回答。逻辑回归和LASSO方法确定，在对生物性别、就业状况、经常光顾的超市和既往旅行进行校正后，食用红肉与携带多重耐药（MDR）大肠杆菌的风险增加相关[比值比（OR）分别为6.13（95%置信区间1.83 - 24.2）和1.82]，而食用鱼类与风险降低相关[OR = 0.27（95%置信区间0.08 - 0.85）和0.81]。
饮食习惯与MDR大肠杆菌粪便携带风险相关。这项研究支持了越来越多的证据，即食物可能是人类共生大肠杆菌中ARGs的重要来源。
