Xu Derui, Song Wenlong, Zhang Jun, Liu Yanting, Lu Yanyan, Zhang Xuewei, Liu Qinyi, Yuan Tianyang, Liu Rui
Department of Spine Surgery, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, Jilin 130040, China.
Department of Spine Surgery, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, Jilin 130040, China.
J Biosci Bioeng. 2021 Aug;132(2):132-139. doi: 10.1016/j.jbiosc.2021.04.006. Epub 2021 May 26.
Polymethyl methacrylate (PMMA) bone cement is a commonly used filling material in orthopedic surgery. Its good and stable performances make it the most widely used in all kinds of bone cement. With the advancement of the application and research of PMMA bone cement by surgeons, its disadvantages such as non-degradation and non-bioactivity are gradually exposed. In recent years, the biological functions of lactoferrin (LF) have been gradually explored, especially its role in promoting osteogenesis. In this study, LF was modified on the surface of solidified PMMA bone cement (LF/PMMA bone cement) by physical/chemical mixed modification and verified by cytological experiments in vitro. In vitro studies have implicated that PMMA bone cement modified with LF can improve the attachment, expansion, proliferation, extracellular matrix secretion and osteogenic differentiation of mouse preosteoblasts (MC3T3-E1) cells, indicating biocompatibility. This experiment provides a novel insight for improving the biological activity of PMMA bone cement and lays a foundation for broadening the clinical application of PMMA bone cement.
聚甲基丙烯酸甲酯（PMMA）骨水泥是骨科手术中常用的填充材料。其良好且稳定的性能使其成为各类骨水泥中应用最为广泛的一种。随着外科医生对PMMA骨水泥应用与研究的推进，其不可降解、无生物活性等缺点逐渐暴露出来。近年来，乳铁蛋白（LF）的生物学功能逐渐被探索，尤其是其在促进成骨方面的作用。在本研究中，通过物理/化学混合改性在固化的PMMA骨水泥表面修饰LF（LF/PMMA骨水泥），并通过体外细胞学实验进行验证。体外研究表明，用LF改性的PMMA骨水泥可改善小鼠前成骨细胞（MC3T3-E1）的附着、伸展、增殖、细胞外基质分泌和成骨分化，表明其具有生物相容性。本实验为提高PMMA骨水泥的生物活性提供了新的见解，为拓宽PMMA骨水泥的临床应用奠定了基础。
