Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Department of Orthopedics, SSL Central Hospital of Dongguan City, Guangdong, China.
Biomed Mater Eng. 2021;32(1):29-37. doi: 10.3233/BME-201139.
Increasing reports on new cement formulations that address the shortcomings of PMMA bone cements and various active components have been introduced to improve the biological activity of PMMA cement.
Evaluating the biological properties of PMMA cements reinforced with Bio-Gene allogeneic bone.
The MC3T3-E1 mouse osteoblast-like cells were utilized to determine the effects of Bio-Gene + PMMA on osteoblast viability, adhesion and differentiation.
The combination of allogeneic bone and PMMA increased the number of adherent live cells compared to both control group and PMMA or Bio-Gene group. Scanning electron microscopy observed that the number of cells adhered to Bio-Gene + PMMA was larger than Bio-Gene and PMMA group. Compared with the control and PMMA or Bio-Gene group, the level of ALP and the number of calcium nodules after osteoinduction was remarkably enhanced in Bio-Gene + PMMA group. Additionally, the combination of Bio-Gene and PMMA induced the protein expression of osteocalcin, osterix and collagen I.
The composition of PMMA and allogeneic bone could provide a more beneficial microenvironment for osteoblast proliferation, adhesion and differentiation. PMMA bone cement reinforced with Bio-Gene allogeneic bone may act as a novel bone substitute to improve the biological activity of PMMA cement.
越来越多的新型水泥配方被报道，这些配方旨在解决 PMMA 骨水泥的缺点和各种活性成分，以提高 PMMA 水泥的生物活性。
评估 Bio-Gene 同种异体骨增强 PMMA 水泥的生物学性能。
采用 MC3T3-E1 小鼠成骨样细胞来确定 Bio-Gene+PMMA 对成骨细胞活力、黏附和分化的影响。
与对照组和 PMMA 组或 Bio-Gene 组相比，同种异体骨与 PMMA 的组合增加了贴壁活细胞的数量。扫描电子显微镜观察到，黏附在 Bio-Gene+PMMA 上的细胞数量多于 Bio-Gene 组和 PMMA 组。与对照组和 PMMA 组或 Bio-Gene 组相比，Bio-Gene+PMMA 组在成骨诱导后 ALP 水平和钙结节数量显著提高。此外，Bio-Gene 和 PMMA 的组合诱导了骨钙素、osterix 和胶原 I 的蛋白表达。
PMMA 和同种异体骨的组合可为成骨细胞的增殖、黏附和分化提供更有利的微环境。Bio-Gene 同种异体骨增强的 PMMA 骨水泥可能作为一种新型骨替代物，提高 PMMA 水泥的生物活性。
