Animal Physiology and Immunology, Technical University of Munich, Freising, Germany.
Animal Physiology and Immunology, Technical University of Munich, Freising, Germany.
J Mol Biol. 2021 Jul 23;433(15):167070. doi: 10.1016/j.jmb.2021.167070. Epub 2021 May 28.
Extracellular vesicles (EVs) are nano-sized, membrane-enclosed vesicles released by cells for intercellular communication. EVs are involved in pathological processes and miRNAs in EVs have gained interest as easily accessible biomolecules in liquid biopsies for diagnostic purposes. To validate potential miRNA biomarker, transcriptome analyses must be carried out to detect suitable reference miRNAs. miREV is a database with over 400 miRNA sequencing data sets and helps the researcher to find suitable reference miRNAs for their individual experimental setup. The researcher can put together a specific sample set in miREV, which is similar to his own experimental concept in order to find the most suitable references. This allows to run validation experiments without having to carry out a complex and costly transcriptome analysis priorly. Additional read count tables of each generated sample set are downloadable for further analysis. miREV is freely available at https://www.physio.wzw.tum.de/mirev/.
细胞外囊泡 (EVs) 是由细胞释放的纳米大小的膜封闭囊泡，用于细胞间通讯。EVs 参与病理过程，EVs 中的 miRNAs 作为液体活检中易于获取的生物分子，已引起人们对其用于诊断目的的兴趣。为了验证潜在的 miRNA 生物标志物，必须进行转录组分析以检测合适的参考 miRNA。miREV 是一个拥有超过 400 个 miRNA 测序数据集的数据库，它可以帮助研究人员找到适合其特定实验设置的参考 miRNA。研究人员可以在 miREV 中组合特定的样本集，与自己的实验概念相似，以找到最合适的参考。这允许在不事先进行复杂和昂贵的转录组分析的情况下运行验证实验。每个生成的样本集的附加读取计数表可下载用于进一步分析。miREV 可在 https://www.physio.wzw.tum.de/mirev/ 免费获取。
