Department of Bioinformatics and Systems Biology, Key Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, PR China.
Huazhong University of Science and Technology Ezhou Industrial Technology Research Institute, Ezhou, Hubei 436044, PR China.
Nucleic Acids Res. 2019 Jan 8;47(D1):D89-D93. doi: 10.1093/nar/gky985.
Extracellular vesicles (EVs), such as exosomes and microvesicles, acted as cell-to-cell communication vectors and potential biomarkers for diseases. microRNAs (miRNAs) are the most well studied molecules in EVs, thus a comprehensive investigation of miRNA expression profiles in EVs will be helpful to explore their functions and biomarkers. We curated 462 small RNA sequencing samples of EVs from 17 sources/diseases and constructed the EVmiRNA database (http://bioinfo.life.hust.edu.cn/EVmiRNA) to show the miRNA expression profiles. We found >1000 miRNAs expressed in these EVs and detected specific miRNAs for EVs of each source/disease. EVmiRNA provides three functional modules: (i) the miRNA expression profiles and the sample information of EVs from different sources (such as blood, breast milk etc.); (ii) the specifically expressed miRNAs in different EVs that would be helpful for biomarker identification; (iii) the miRNA annotations including the miRNA expression in EVs and TCGA cancer types, miRNA pathway regulations as well as miRNA function and publications. EVmiRNA has a user-friendly web interface with powerful browse and search functions, as well as data downloading. It is the first database focusing on miRNA expression profiles in EVs and will be useful for the research and application community of EV biomarker, miRNA function and liquid biopsy.
细胞外囊泡（EVs），如外泌体和微囊泡，充当细胞间通讯载体和疾病的潜在生物标志物。microRNAs（miRNAs）是 EVs 中研究最多的分子，因此对 EVs 中 miRNA 表达谱进行全面研究有助于探索其功能和生物标志物。我们整理了来自 17 个来源/疾病的 462 个 EVs 的小 RNA 测序样本，并构建了 EVmiRNA 数据库（http://bioinfo.life.hust.edu.cn/EVmiRNA）以展示 miRNA 表达谱。我们发现这些 EVs 中表达了 >1000 个 miRNAs，并检测到了每个来源/疾病的 EV 特异性 miRNAs。EVmiRNA 提供了三个功能模块：（i）来自不同来源（如血液、母乳等）的 EVs 的 miRNA 表达谱和样本信息；（ii）不同 EVs 中特异性表达的 miRNAs，有助于生物标志物的识别；（iii）miRNA 注释，包括 EVs 中的 miRNA 表达、TCGA 癌症类型、miRNA 通路调控以及 miRNA 功能和出版物。EVmiRNA 具有用户友好的 Web 界面，具有强大的浏览和搜索功能，以及数据下载功能。它是第一个专注于 EVs 中 miRNA 表达谱的数据库，将有助于 EV 生物标志物、miRNA 功能和液体活检的研究和应用社区。
