Jiang Min, Zhang Fen, Yuan Qiao, Lin Peixing, Zheng Hao, Liang Sha, Jian Yue, Miao Huiying, Li Huanxiu, Wang Qiaomei, Sun Bo
College of Horticulture, Sichuan Agricultural University, Chengdu, China.
Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou, China.
Front Plant Sci. 2021 May 14;12:662684. doi: 10.3389/fpls.2021.662684. eCollection 2021.
Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing , the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained -silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in -silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, -silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of provides insight for the biosynthesis and regulation of carotenoid in Chinese kale.
类胡萝卜素是有机色素，在植物着色和人类健康方面都发挥着重要作用；由于食品和医药领域对天然分子的需求不断增长，它们成为分子育种中的关键研究对象。在本研究中，我们重点对类胡萝卜素生物合成途径分支之前的类胡萝卜素异构酶基因进行表征，该基因在中国芥蓝的所有器官和发育阶段均有表达，以及位于叶绿体中的BoaCRTISO。该基因的表达受强光、红蓝光组合光和赤霉素处理诱导，但受黑暗和脱落酸处理抑制。我们通过病毒诱导基因沉默技术获得了该基因沉默的植株，沉默效率在52%至77%之间。该基因沉默植株中大多数类胡萝卜素和叶绿素生物合成基因的表达下调，类胡萝卜素和叶绿素含量降低。同时，该基因沉默植株表现出叶片发黄和生长受抑制的表型。该基因的功能表征为中国芥蓝中类胡萝卜素的生物合成和调控提供了见解。
