Talesh Sasani Soheila, M Soltani Bahram, Mehrabi Rahim, Fereidoun Padasht-Dehkaei Hossein Samavatian
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Biology, University of Guilan, Rasht, Iran.
Iran J Biotechnol. 2020 Oct 1;18(4):e2451. doi: 10.30498/IJB.2020.2451. eCollection 2020 Oct.
MicroRNAs, as small non-coding RNAs, are recently reported to be involved in plant defense system against pathogens including fungi.
In this research, it was intended to investigate candidate susceptible rice (Oryza Sativa) Osa-miRNA expression alteration following the infection by .
To this aim, literature review suggested eight conserved plant miRNAs that are involved in other plant-pathogen interactions. Then, sixty days old rice plants (Hashemi, susceptible cultivar) were inoculated with and candidate miRNA expression alterations were investigated 2 hpi (hours post inoculation), 2 dpi (days post inoculation) and 6 dpi.
RT-qPCR analysis suggested four subgroups of candidate miRNAs based on the time of their responses to the pathogenesis of . While Osa-miR-156 was early-responsive, Osa-miR159 was the last-responsive and Osa-miR167, Osa-miR171, Osa-miR408, and Osa-miR444 were late responsive to infection. and were non-responsive to this infection, compared to the mock-inoculated control group. Consistently, Os-SPL3 and Os-MADS known target genes were expressed in reverse correlation to Osa-miR156 and Osa-miR444, respectively.
From these data, it is suggested that both early (Osa-miR-156) and late (Osa-miR167, Osa-miR171, Osa- miR408, Osa-miR444) responsive miRNAs might be involved in infection in rice plants.
微小RNA作为小型非编码RNA，最近有报道称其参与植物针对包括真菌在内的病原体的防御系统。
本研究旨在调查候选易感水稻（Oryza Sativa）Osa-miRNA在感染后表达的变化。
为此，文献综述提出了8种参与其他植物-病原体相互作用的保守植物微小RNA。然后，用接种60天龄的水稻植株（Hashemi，易感品种），并在接种后2小时（hpi）、2天（dpi）和6天调查候选微小RNA表达的变化。
RT-qPCR分析基于候选微小RNA对发病机制的反应时间，提出了四个亚组。虽然Osa-miR-156是早期反应型，Osa-miR159是最后反应型，而Osa-miR167、Osa-miR171、Osa-miR408和Osa-miR444对感染反应较晚。与模拟接种的对照组相比，和对这种感染无反应。一致地，已知的靶基因Os-SPL3和Os-MADS分别与Osa-miR156和Osa-miR444呈反向表达。
从这些数据表明，早期反应型（Osa-miR-156）和晚期反应型（Osa-miR167、Osa-miR171、Osa-miR408、Osa-miR444）的微小RNA可能都参与了水稻植株的感染。
