拟南芥高度重复序列的特征分析

Simoens C R, Gielen J, Van Montagu M, Inzé D

Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.

Nucleic Acids Res. 1988 Jul 25;16(14B):6753-66. doi: 10.1093/nar/16.14.6753.

We have analyzed three classes of highly repetitive DNA sequences of Arabidopsis thaliana, composed of tamdemly repeated units of 180 bp, 500 bp, and 160 bp, respectively. The three families comprise approximately 2% of the Arabidopsis genome and are the major component of the highly repetitive DNA. The 500-bp element arose by duplication of one half of a 180-bp ancestor and insertion of a foreign segment between the two duplicated parts followed by amplification. The repeat elements contain occasionally palindromes and other motifs but none are significantly conserved. There is no significant similarity with previously published repetitive elements. Heterogeneity between monomers ranges from 6% to 17%. Monomers derived from different clusters in the genome are more diverged than monomers of the same array.

我们分析了拟南芥的三类高度重复DNA序列，它们分别由180 bp、500 bp和160 bp的串联重复单元组成。这三个家族约占拟南芥基因组的2%，是高度重复DNA的主要组成部分。500 bp的元件是由一个180 bp祖先的一半复制而来，在两个复制部分之间插入一个外源片段，随后进行扩增。这些重复元件偶尔包含回文结构和其他基序，但没有一个是显著保守的。与先前发表的重复元件没有显著相似性。单体之间的异质性范围为6%至17%。来自基因组中不同簇的单体比同一阵列中的单体差异更大。