Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, 315211, People's Republic of China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China.
Arch Microbiol. 2021 Sep;203(7):4141-4148. doi: 10.1007/s00203-021-02404-5. Epub 2021 May 31.
Lactobacillus plantarum is one of common probiotics in fermented foods. Quorum sensing (QS) is a common communication way within bacteria. It is not clear whether the probiotic properties of L. plantarum mediated by QS. Here, Lb. plantarum YM-4-3 was examined for resistance of pH, bile, antimicrobial and luxS gene expression pattern. The study found that: (1) the supernatant of YM-4-3 had bacteriostatic effect to Escherichia coli O157:H7, Listeria monocytogenes and Staphylococcus aureus; (2) Lb. plantarum YM-4-3 shown tolerance property to the strongest acid culture that pH value of 3; (3) the bile tolerance of Lb. plantarum YM-4-3 was significant difference with the growth stage, the early exponential phase of the growth culture can tolerate bile of 0.4% (w/v), while the stationary growth stage can only tolerate bile of 0.2%; (4) Lb. plantarum YM-4-3 luxS gene was contrary expression along with the growth. (5) Compared with the wild-type strain, the adhesion ability of Lb. plantarum YM-4-3 ΔluxS was decreased obviously. These results showed that AI-2 LuxS quorum sensing system mediating Lb. plantarum acid, bile tolerance, antimicrobial and adhesion of probiotics.
植物乳杆菌是发酵食品中常见的益生菌之一。群体感应 (QS) 是细菌内部常见的一种通讯方式。目前尚不清楚 QS 是否介导了植物乳杆菌的益生菌特性。本研究以植物乳杆菌 YM-4-3 为研究对象，考察了其对 pH 值、胆汁、抗菌物质和 luxS 基因表达模式的耐受性。研究发现：（1）YM-4-3 的上清液对大肠杆菌 O157:H7、单核细胞增生李斯特菌和金黄色葡萄球菌具有抑菌作用；（2）植物乳杆菌 YM-4-3 对 pH 值为 3 的最强酸性培养物表现出耐受性；（3）植物乳杆菌 YM-4-3 的胆汁耐受性与生长阶段有显著差异，生长培养的早期指数期可耐受 0.4%（w/v）的胆汁，而静止生长期仅能耐受 0.2%的胆汁；（4）植物乳杆菌 YM-4-3 luxS 基因的表达与生长相反。（5）与野生型菌株相比，植物乳杆菌 YM-4-3ΔluxS 的黏附能力明显降低。这些结果表明，AI-2 LuxS 群体感应系统介导了植物乳杆菌的耐酸、耐胆汁、抗菌和黏附等益生菌特性。
