乳酸菌拮抗作用的筛选与代谢组学分析

Screening and Metabolomic Analysis of Lactic Acid Bacteria-Antagonizing .

作者信息

Li Jianzhou, Chen Xiaohua, Xie Ziyan, Liang Lin, Li Anping, Zhao Chao, Wen Yuxi, Lou Zaixiang

机构信息

College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.

Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences, Hengyang Normal University, Hengyang 421008, China.

出版信息

Foods. 2023 Jul 23;12(14):2799. doi: 10.3390/foods12142799.

DOI:10.3390/foods12142799

PMID:37509891

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10379379/

Abstract

is a conditional Gram-negative pathogen that produces extracellular virulence factors that can lead to bloodstream invasion, severely harm tissues, and disseminate bacteria, ultimately leading to various diseases. In this study, lactic acid bacteria (LAB) with strong antagonistic ability against were screened, and the regulatory mechanism of LAB against was evaluated. The results showed that the three selected LAB strains had strong inhibition ability on the growth, biofilm formation, and pyocyanin expression of and a promoting effect on the expression of autoinducer-2. Among them, LPyang is capable of affecting the metabolic processes of by influencing metabolic substances, such as LysoPC, oxidized glutathione, betaine, etc. These results indicate that LPyang reduces the infectivity of through inhibition of its growth, biofilm formation, pyocyanin expression, and regulation of its metabolome. This study provides new insights into the antagonistic activity of LPyang against .

摘要

是一种条件性革兰氏阴性病原体，可产生细胞外毒力因子，导致血流侵袭、严重损害组织并传播细菌，最终引发各种疾病。在本研究中，筛选出了对具有强拮抗能力的乳酸菌（LAB），并评估了LAB对的调控机制。结果表明，所选的三株LAB菌株对的生长、生物膜形成和绿脓菌素表达具有较强的抑制能力，并对自诱导物-2的表达有促进作用。其中，LPyang能够通过影响溶血磷脂酰胆碱、氧化型谷胱甘肽、甜菜碱等代谢物质来影响的代谢过程。这些结果表明，LPyang通过抑制的生长、生物膜形成、绿脓菌素表达及其代谢组调控来降低其感染性。本研究为LPyang对的拮抗活性提供了新的见解。