College of Life Science and Technology, Henan Engineering Research Center of Crop Genome Editing, Henan Institute of Science and Technology, Xinxiang 453003, China.
College of Life Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang 453003, China.
Phytopathology. 2021 Dec;111(12):2309-2316. doi: 10.1094/PHYTO-11-20-0506-R. Epub 2021 Dec 1.
Fusarium head blight (FHB), mainly caused by , has become one of the most serious diseases that damage wheat. The (pore-forming toxin-like) and (histidine-rich calcium-binding protein) genes at the quantitative trait locus were identified to confer resistance to FHB in the wheat cultivar Sumai 3. In this study, a wheat ricin B-like lectin gene (designated ) that interacted with TaPFT was isolated by a yeast two-hybrid screen of a wheat cDNA library. A yeast two-hybrid and bimolecular fluorescence complementation study further verified that TaRBL interacted with TaPFT but not with TaHRC. Gene expression studies showed that upon infection, expression was upregulated in resistant cultivars but downregulated in susceptible cultivars. Furthermore, knockdown of expression by barley stripe mosaic virus-induced gene silencing significantly reduced the resistance of wheat to FHB in both the resistant cultivar Sumai 3 and the susceptible cultivar Jimai 22. Thus, we conclude that encodes a ricin B-like lectin protein that interacts with TaPFT and is involved in resistance to FHB in wheat.
镰刀菌穗腐病（FHB）主要由 引起，已成为危害小麦的最严重疾病之一。在小麦品种苏麦 3 中，已鉴定出数量性状位点上的 （孔形成毒素样）和 （富含组氨酸的钙结合蛋白）基因赋予其对 FHB 的抗性。本研究通过小麦 cDNA 文库的酵母双杂交筛选，分离到一个与 TaPFT 互作的小麦蓖麻 B 型凝集素基因（命名为 ）。酵母双杂交和双分子荧光互补实验进一步验证了 TaRBL 与 TaPFT 相互作用，但不与 TaHRC 相互作用。基因表达研究表明，在 感染后，抗性品种中的 表达上调，而感病品种中的表达下调。此外，通过大麦黄花叶病毒诱导的基因沉默敲低 的表达，显著降低了苏麦 3 和感病品种济麦 22 对 FHB 的抗性。因此，我们得出结论， 编码一种与 TaPFT 相互作用的蓖麻 B 型凝集素蛋白，参与小麦对 FHB 的抗性。
