泊松分布统计在离子通道重建中用于确定寡聚体结构的应用。
The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture.
机构信息
Program in Biophysics, University of Michigan, Ann Arbor, MI, United States; Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States.
出版信息
Methods Enzymol. 2021;652:321-340. doi: 10.1016/bs.mie.2021.02.018. Epub 2021 Mar 27.
During reconstitution, membrane proteins are randomly inserted into liposomes according to Poisson distribution statistics. When the protein to lipid ratios in the reconstitution mixture are varied systematically, the characteristics of this statistical capture permit inferences about the proteins themselves, such as the number of subunits that assemble into a single functional unit. This chapter describes the Poisson distribution as applied to the reconstitution of membrane proteins into proteoliposomes and focuses on an application whereby this statistical behavior is used to determine the number of ion channel subunits that assemble into a functional pore. Practical considerations for performing these experiments are emphasized. Harnessing Poisson dilution statistics provides a function-based method to determine ion channel oligomerization, complementing other biophysical, biochemical, or structural approaches.
在复性过程中，膜蛋白根据泊松分布统计随机插入脂质体。当复性混合物中的蛋白与脂质比例被系统改变时，这种统计捕获的特点允许对蛋白质本身进行推断，例如组装成单个功能单元的亚基数量。本章介绍了泊松分布在将膜蛋白复性为脂蛋白体中的应用，并重点介绍了一种应用，即通过这种统计行为来确定组装成功能性孔的离子通道亚基数量。强调了进行这些实验的实际考虑因素。利用泊松稀释统计提供了一种基于功能的方法来确定离子通道寡聚化，补充了其他生物物理、生化或结构方法。
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