海枣（Phoenix dactylifera L.）的离体植株再生系统：螯合铁源的影响

In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources.

作者信息

Al-Mayahi Ahmed Madi Waheed

机构信息

Date Palm Research Centre, University of Basrah, Basrah, Iraq.

出版信息

J Genet Eng Biotechnol. 2021 Jun 1;19(1):83. doi: 10.1186/s43141-021-00177-4.

DOI:10.1186/s43141-021-00177-4

PMID:34061279

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8169728/

Abstract

BACKGROUND

Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study's objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar.

RESULTS

The addition of FeEDDHA to the culture medium was more effective than FeEDTA on callus growth, shoot regeneration, and the number of shoots per jar, where the best result (220.8mg callus, 86.67% and 17.2 shoots per jar, respectively) was obtained by using 93.5 mg L FeEDDHA (5.6 mg L Fe), compared with other treatments. The results also indicate that using 93.5 mg L FeEDDHA (5.6 mg L Fe) as a supplement can decrease antioxidant enzymes CAT and POD activity compared to the rest of the treatments. Medium equipped with 187.0 mg L FeEDDHA (11.2 mg LFe) had the highest rooting percentage and number of roots per shoot than other treatments. The biochemical analysis results showed that treatments with FeEDDHA of 280.5 mg L (16.8 mg L Fe) and 187.0 mg L (11.2 mg LFe) significantly increased the iron content. The results showed that shoot maximum chlorophyll and endogenous IAA level content were recorded in a medium supplemented with 187.0 mg L FeEDDHA (11.2 mg LFe) as Fe source.

CONCLUSION

FeEDDHA used in the present study was proven to be a promising iron chelate source in comparison with the FeEDTA sources.

摘要

背景

铁螯合物来源及其浓度是枣椰树离体繁殖的重要因素。本研究的目的是研究铁螯合形式对Barhee品种组织培养生长发育的影响。

结果

在培养基中添加FeEDDHA对愈伤组织生长、芽再生和每瓶芽数的效果比FeEDTA更好，与其他处理相比，使用93.5 mg/L FeEDDHA（5.6 mg/L Fe）时获得了最佳结果（分别为220.8mg愈伤组织、86.67%和每瓶17.2个芽）。结果还表明，与其他处理相比，使用93.5 mg/L FeEDDHA（5.6 mg/L Fe）作为补充剂可降低抗氧化酶CAT和POD的活性。配备187.0 mg/L FeEDDHA（11.2 mg/L Fe）的培养基比其他处理具有最高的生根率和每枝根数。生化分析结果表明，280.5 mg/L（16.8 mg/L Fe）和187.0 mg/L（11.2 mg/L Fe）的FeEDDHA处理显著增加了铁含量。结果表明，以187.0 mg/L FeEDDHA（11.2 mg/L Fe）作为铁源的培养基中，芽的叶绿素和内源IAA水平最高。