Hubei Provincial Engineering Laboratory for Pond Aquaculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Departments of Chemistry and Biology, Carleton University, Ottawa, Ontario K1S 5B6, Canada.
Environ Sci Technol. 2021 Jun 15;55(12):8108-8118. doi: 10.1021/acs.est.0c07708. Epub 2021 Jun 1.
Tris(1,3-dichloro-2-propyl)phosphate (TDCIPP) has commonly been used as an additive flame retardant and frequently detected in the aquatic environment and in biological samples worldwide. Recently, it was found that exposure to TDCIPP inhibited the growth of zebrafish, but the relevant molecular mechanisms remained unclear. In this study, 5 day-old crucian carp () larvae were treated with 0.5, 5, or 50 μg/L TDCIPP for 90 days; the effect on growth was evaluated; and related molecular mechanisms were explored. Results demonstrated that 5 or 50 μg/L TDCIPP treatment significantly inhibited the growth of crucian carp and downregulated the expression of growth hormones (), growth hormone receptor (), and insulin-like growth factor 1 (). Molecular docking, dual-luciferase reporter gene assay, and experiments demonstrated that TDCIPP could bind to the growth hormone releasing hormone receptor protein of crucian carp and disturb the stimulation of growth hormone releasing hormone to the expression of , resulting in the decrease of the mRNA level of and in pituitary cells. Our findings provide new perceptions into the molecular mechanisms of developmental toxicity of TDCIPP in fish.
磷酸三(1,3-二氯丙基)酯（TDCIPP）通常被用作添加剂型阻燃剂，在全球范围内的水生环境和生物样本中经常被检测到。最近发现，暴露于 TDCIPP 会抑制斑马鱼的生长，但相关的分子机制仍不清楚。在这项研究中，用 0.5、5 或 50μg/L 的 TDCIPP 处理 5 天大的鲫鱼（）幼虫 90 天；评估对生长的影响；并探讨相关的分子机制。结果表明，5 或 50μg/L 的 TDCIPP 处理显著抑制了鲫鱼的生长，并下调了生长激素（）、生长激素受体（）和胰岛素样生长因子 1（）的表达。分子对接、双荧光素酶报告基因检测和 实验表明，TDCIPP 可以与鲫鱼的生长激素释放激素受体蛋白结合，并干扰生长激素释放激素对 的表达的刺激，导致垂体细胞中 和 的 mRNA 水平降低。我们的研究结果为 TDCIPP 对鱼类发育毒性的分子机制提供了新的认识。
