College of Fisheries, Huazhong Agricultural University , Wuhan 430070, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210089, China.
Environ Sci Technol. 2015 Dec 15;49(24):14579-87. doi: 10.1021/acs.est.5b03849. Epub 2015 Nov 12.
Bioconcentrations of tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) in brain, gonad, and liver as well as effects on fecundity and development of zebrafish (Danio rerio) were determined. Zebrafish (1-month old) were exposed to environmentally relevant concentrations of 29 ± 2.1, 600 ± 21, or 6300 ± 130 ng TDCIPP/L. After 120 days of exposure, TDCIPP accumulated in the brain, gonad, and liver with bioconcentration factors of 460, 38, and 87 in females and 26, 55, and 110 in males, respectively. TDCIPP accumulated to a greater extent in brains of females than those of males. Exposure to 6300 ± 130 ng TDCIPP/L resulted in significantly (P < 0.05) fewer eggs being produced, but the histology of the gonad, plasma concentrations of estradiol and 11-ketotestosterone, and expression of genes involved in hypothalamic-pituitary-gonadal-liver axis were not significantly (P > 0.05) different between individuals exposed to TDCIPP and the unexposed control fish. Exposure to TDCIPP resulted in shorter body length, lighter body mass, and lower gonadal-somatic index in females. These effects were possibly due to down-regulation of expression of genes along the growth hormone/insulin-like growth factor (GH/IGF) axis. Correlations between the production of eggs and developmental parameters or expression of genes along the GH/IGF axis further suggested that environmentally relevant concentrations of TDCIPP could have adverse effects on reproduction, possibly due to the inhibition of the growth of females.
测定了三（1,3-二氯-2-丙基）磷酸酯（TDCIPP）在大脑、性腺和肝脏中的生物浓缩以及对斑马鱼（Danio rerio）生殖力和发育的影响。将斑马鱼（1 个月大）暴露于环境相关浓度为 29 ± 2.1、600 ± 21 或 6300 ± 130 ng TDCIPP/L 的环境中。暴露 120 天后，TDCIPP 在雌性鱼的大脑、性腺和肝脏中的生物浓缩因子分别为 460、38 和 87，在雄性鱼中分别为 26、55 和 110。TDCIPP 在雌性鱼的大脑中积累的程度比雄性鱼更大。暴露于 6300 ± 130 ng TDCIPP/L 导致产卵量显著减少（P < 0.05），但性腺组织学、血浆雌二醇和 11-酮睾酮浓度以及参与下丘脑-垂体-性腺-肝轴的基因表达在暴露于 TDCIPP 的个体与未暴露的对照鱼之间无显著差异（P > 0.05）。TDCIPP 暴露导致雌性鱼的体长变短、体重变轻、性腺-体指数降低。这些影响可能是由于生长激素/胰岛素样生长因子（GH/IGF）轴基因表达下调所致。产卵量与生长激素/胰岛素样生长因子（GH/IGF）轴发育参数或基因表达之间的相关性进一步表明，环境相关浓度的 TDCIPP 可能对生殖产生不利影响，这可能是由于雌性生长受到抑制。
