Padmathilake Kaluhannadige R E, Fernando Wannakuwattewaduge Gerard Dilantha
Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Int J Mol Sci. 2022 Apr 2;23(7):3964. doi: 10.3390/ijms23073964.
causes blackleg disease, which is one of the most destructive diseases of canola ( L.). Due to the erosion of the current resistance in , it is pivotal to introduce new resistant genotypes to the growers. This study evaluated the potential of gene as resistance to its corresponding avirulence gene is abundant. The line was inoculated with isolate with ; UMAvr7; and the CRISPR/Cas9 knockout mutant, umavr7, of the same isolate to cause incompatible and compatible interactions, respectively. Dual RNA-seq showed differential gene expressions in both interactions. High expressions of virulence-related pathogen genes-CAZymes, merops, and effector proteins after 7-dpi in compatible interactions but not in incompatible interaction-confirmed that the pathogen was actively virulent only in compatible interactions. Salicyclic and jasmonic acid biosynthesis and signaling-related genes, defense-related gene (GSBRNA2T00150001001), and GSBRNA2T00068522001 in the NLR gene family were upregulated starting as early as 1- and 3-dpi in the incompatible interaction and the high upregulation of those genes after 7-dpi in compatible interactions confirmed the early recognition of the pathogen by the host and control it by early activation of host defense mechanisms in the incompatible interaction.
引发黑腿病，黑腿病是油菜（L.）最具毁灭性的病害之一。由于当前油菜品种的抗性正在减弱，因此向种植者引入新的抗性基因型至关重要。本研究评估了基因的潜力，因为其对应的无毒基因很丰富。用菌株接种油菜品系，该菌株带有；UMAvr7；以及同一菌株的CRISPR/Cas9敲除突变体umavr7，分别引发不亲和和亲和互作。双RNA测序显示在两种互作中基因表达存在差异。在亲和互作中接种7天后，与毒力相关的病原体基因——碳水化合物活性酶、默洛普蛋白酶和效应蛋白高表达，但在不亲和互作中则不然，这证实病原体仅在亲和互作中具有活跃的毒力。水杨酸和茉莉酸生物合成及信号传导相关基因、防御相关基因（GSBRNA2T00150001001）以及NLR基因家族中的GSBRNA2T00068522001在不亲和互作中最早在接种1天和3天后上调，且在亲和互作中接种7天后这些基因高度上调，这证实了宿主对病原体的早期识别，并通过在不亲和互作中早期激活宿主防御机制来控制病原体。
