Liang Panhong, Li Yanping, Mao Li, Liu Tingli, Zhang Shaohua, Ehsan Muhammad, Wang Liqun, Guo Aimin, Chen Guoliang, Luo Xuenong
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Nanjing 210014, China.
Microorganisms. 2021 May 5;9(5):994. doi: 10.3390/microorganisms9050994.
is a zoonotic parasite found in the human intestine and pig liver that evolved various strategies to survive the host's defenses. Exosomes are membranous vesicles released by cells and are an important vehicle in parasite-host interactions. However, no literature exists on the specific infection mechanisms of against the host defense response, and further research is required to understand the parasite-host interaction. In this study, we investigated the host's differentially expressed genes (DEGs) while stimulating them with exosomes derived from the adult worm (Tas-exo) on LoVo by RNA-seq analysis. Our results identified 348 genes as being significantly differentially expressed for the Tas-exo group when comparing with that of the NC group. Some of these genes are related to modulation of cell proliferation and cell autophagy. Surprisingly, autophagy and cell proliferation have crucial roles in the defense against parasites; accordingly, we detected cell proliferation and autophagy in LoVo cells by CCK8, immunofluorescence, and Western blotting, demonstrating that Tas-exo could inhibit LoVo cell proliferation and autophagy via AMPK pathway. When P62 and p-mTOR/mTOR expression were significantly increased, BeclinI and pAMPK/AMPK were significantly decreased. These results expand our understanding of parasite-host interactions mediated by exosomes.
是一种人畜共患寄生虫，存在于人体肠道和猪肝中，它进化出了多种策略来抵御宿主的防御。外泌体是细胞释放的膜性囊泡，是寄生虫与宿主相互作用中的重要载体。然而，关于其针对宿主防御反应的具体感染机制尚无文献报道，需要进一步研究以了解寄生虫与宿主的相互作用。在本研究中，我们通过RNA测序分析，在用来自成虫的外泌体（Tas-exo）刺激LoVo细胞时，研究了宿主的差异表达基因（DEG）。我们的结果表明，与NC组相比，Tas-exo组有348个基因显著差异表达。其中一些基因与细胞增殖和细胞自噬的调节有关。令人惊讶的是，自噬和细胞增殖在抵御寄生虫方面起着关键作用；因此，我们通过CCK8、免疫荧光和蛋白质印迹法在LoVo细胞中检测了细胞增殖和自噬，证明Tas-exo可通过AMPK途径抑制LoVo细胞增殖和自噬。当P62和p-mTOR/mTOR表达显著增加时，BeclinI和pAMPK/AMPK显著降低。这些结果扩展了我们对外泌体介导的寄生虫与宿主相互作用的理解。
