Jemeršić Lorena, Lojkić Ivana, Krešić Nina, Keros Tomislav, Zelenika Tajana Amšel, Jurinović Luka, Skok Damir, Bata Ingeborg, Boras Jadranko, Habrun Boris, Brnić Dragan
Virology Department, Croatian Veterinary Institute, Savska Cesta 143, 10000 Zagreb, Croatia.
Poultry Center, Croatian Veterinary Institute, Heinzelova 55, 10000 Zagreb, Croatia.
Pathogens. 2021 May 21;10(6):635. doi: 10.3390/pathogens10060635.
Due to SARS CoV-2 recombination rates, number of infected people and recent reports of environmental contamination, the possibility of SARS CoV-2 transmission to animals can be expected. We tested samples of dominant free-living and captive wildlife species in Croatia for the presence of anti-SARS CoV-2 antibodies and viral RNA. In total, from June 2020 until February 2021, we tested blood, muscle extract and fecal samples of 422 free-living wild boars (), red foxes () and jackals (); blood and cloacal swabs of 111 yellow-legged gulls () and fecal samples of 32 zoo animals. A commercially available ELISA (ID.Vet, France) and as a confirmatory test, a surrogate virus neutralization test (sVNT; GenScript, Netherlands) were used. Fecal samples were tested for the presence of viral RNA by a real-time RT-PCR protocol. Fifteen out of 533 (2.8%) positive ELISA results were detected; in wild boars (3.9%), red foxes (2.9%) and jackals (4.6%). However, the positive findings were not confirmed by sVNT. No viral RNA was found. In conclusion, no spillover occurred within the investigated period (second COVID-19 wave). However, further investigation is needed, especially regarding wildlife sample features for serological tests.
由于严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的重组率、感染人数以及近期有关环境污染的报道，可以预期SARS-CoV-2有传播给动物的可能性。我们检测了克罗地亚主要的自由生活和圈养野生动物物种的样本，以检测抗SARS-CoV-2抗体和病毒RNA的存在情况。从2020年6月到2021年2月，我们总共检测了422只自由生活的野猪、赤狐和胡狼的血液、肌肉提取物和粪便样本；111只黄腿鸥的血液和泄殖腔拭子以及32只动物园动物的粪便样本。使用了一种市售的酶联免疫吸附测定法（ELISA，法国ID.Vet公司），并作为确证试验，使用了替代病毒中和试验（sVNT，荷兰金斯瑞公司）。通过实时逆转录聚合酶链反应（RT-PCR）方案检测粪便样本中病毒RNA的存在情况。在533份ELISA阳性结果中检测到15份（2.8%）；在野猪（3.9%）、赤狐（2.9%）和胡狼（4.6%）中检测到。然而，sVNT未证实这些阳性结果。未发现病毒RNA。总之，在调查期间（第二波新冠疫情）未发生病毒溢出。然而，需要进一步调查，特别是关于血清学检测的野生动物样本特征。
