Mustonen Anne-Mari, Käkelä Reijo, Joukainen Antti, Lehenkari Petri, Jaroma Antti, Kääriäinen Tommi, Kröger Heikki, Paakkonen Tommi, Sihvo Sanna P, Nieminen Petteri
Institute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland.
Department of Environmental and Biological Sciences, Faculty of Science and Forestry, University of Eastern Finland, P.O. Box 111, FI-80101 Joensuu, Finland.
Biology (Basel). 2021 May 4;10(5):401. doi: 10.3390/biology10050401.
Anomalies of fatty acid (FA) metabolism characterize osteoarthritis (OA) and rheumatoid arthritis (RA) in the knee joint. No previous study has investigated the synovial fluid (SF) FA manifestations in these aging-related inflammatory diseases in the shoulder. The present experiment compared the FA alterations between the shoulder and knee joints in patients with end-stage OA or end-stage RA. SF samples were collected during glenohumeral or knee joint surgery from trauma controls and from OA and RA patients (n = 42). The FA composition of SF total lipids was analyzed by gas chromatography with flame ionization and mass spectrometric detection and compared across cohorts. The FA signatures of trauma controls were mostly uniform in both anatomical locations. RA shoulders were characterized by elevated percentages of 20:4n-6 and 22:6n-3 and with reduced proportions of 18:1n-9. The FA profiles of OA and RA knees were relatively uniform and displayed lower proportions of 18:2n-6, 22:6n-3 and total n-6 polyunsaturated FAs (PUFAs). The results indicate location- and disease-dependent differences in the SF FA composition. These alterations in FA profiles and their potential implications for the production of PUFA-derived lipid mediators may affect joint lubrication, synovial inflammation and pannus formation as well as cartilage and bone degradation and contribute to the pathogeneses of inflammatory joint diseases.
脂肪酸（FA）代谢异常是膝关节骨关节炎（OA）和类风湿关节炎（RA）的特征。此前尚无研究调查这些与衰老相关的肩部炎症性疾病中的滑液（SF）脂肪酸表现。本实验比较了终末期OA或终末期RA患者肩部和膝关节之间的脂肪酸变化。在肩关节或膝关节手术期间，从创伤对照组以及OA和RA患者（n = 42）中采集滑液样本。通过带有火焰离子化和质谱检测的气相色谱法分析滑液总脂质的脂肪酸组成，并在各队列之间进行比较。创伤对照组的脂肪酸特征在两个解剖部位大多是一致的。RA肩部的特征是20:4n-6和22:6n-3的百分比升高，而18:1n-9的比例降低。OA和RA膝关节的脂肪酸谱相对一致，且18:2n-6、22:6n-3和总n-6多不饱和脂肪酸（PUFA）的比例较低。结果表明滑液脂肪酸组成存在部位和疾病依赖性差异。脂肪酸谱的这些变化及其对PUFA衍生脂质介质产生的潜在影响可能会影响关节润滑、滑膜炎症和血管翳形成以及软骨和骨降解，并有助于炎症性关节疾病的发病机制。
