Bang Chul Hwan, Song Ji Young, Song Yu Mee, Lee Ji Hyun, Park Young Min, Lee Jun Young
Department of Dermatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, Korea.
Program of Immunology & Microbiology, Department of Biomedicine & Health Science, Graduate School, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, Korea.
J Clin Med. 2021 May 4;10(9):1976. doi: 10.3390/jcm10091976.
IL-31 is involved in pruritus in atopic dermatitis (AD) and the pathogenesis of AD. However, the mechanism of IL-31 production is not fully understood. We sought to investigate the association between CD45ROCLAH4R T cells and IL-31 production. Immunofluorescence studies were performed retrospectively on punch-biopsy specimens from five people with AD and three healthy controls. In addition, blood samples were collected prospectively from eight patients with AD and eight healthy controls for sorting CD45ROCLAH4R T cells. There was no overlap of patients between the biopsy group and the blood sampling group. Sorted cells were stimulated with 4-methylhistamine (4MH), and the level of IL-31 was measured by an enzyme-linked immunosorbent assay. Immunofluorescence showed co-localization of H4R and IL-31 in lesional AD skin but not in normal skin of healthy controls. The proportion of CLAH4R T cells among CD3CD45RO lymphocytes was 18.3 ± 6.2% in patients with AD and 11.2 ± 7.6% in healthy controls. In the AD group, production of IL-31 by CD45ROCLAH4R T cells increased from 32.4 ± 13.3 pg/mL to 47.5 ± 18.7 pg/mL by 4MH stimulation after 24 h ( < 0.001). However, in the control group, production of IL-31 was 20.1 ± 10.6 pg/mL without and 22.1 ± 9.3 pg/mL with 4MH stimulation ( > 0.05). According to our study, CD45ROCLAH4R T cells are an important source of IL-31 in AD, and may be a target for treatment of IL-31-induced pruritus.
白细胞介素-31（IL-31）参与特应性皮炎（AD）的瘙痒及AD的发病机制。然而，IL-31产生的机制尚未完全明确。我们试图研究CD45ROCLA⁺H4R⁺ T细胞与IL-31产生之间的关联。对来自5例AD患者和3名健康对照的打孔活检标本进行回顾性免疫荧光研究。此外，前瞻性收集8例AD患者和8名健康对照的血样，用于分选CD45ROCLA⁺H4R⁺ T细胞。活检组和血样采集组之间没有患者重叠。分选后的细胞用4-甲基组胺（4MH）刺激，通过酶联免疫吸附测定法测量IL-31水平。免疫荧光显示H4R和IL-31在AD皮损皮肤中共定位，但在健康对照的正常皮肤中未共定位。AD患者中CD3⁺CD45RO⁺淋巴细胞中CLA⁺H4R⁺ T细胞的比例为18.3±6.2%，健康对照中为11.2±7.6%。在AD组中，CD45ROCLA⁺H4R⁺ T细胞产生的IL-31在24小时经4MH刺激后从32.4±13.3 pg/mL增加到47.5±18.7 pg/mL（P<0.001）。然而，在对照组中，未刺激时IL-31产生量为20.1±10.6 pg/mL，4MH刺激后为22.1±9.3 pg/mL（P>0.05）。根据我们的研究，CD45ROCLA⁺H4R⁺ T细胞是AD中IL-31的重要来源，可能是治疗IL-31诱导瘙痒的靶点。
