Doan Chien Thang, Chen Chien-Lin, Nguyen Van Bon, Tran Thi Ngoc, Nguyen Anh Dzung, Wang San-Lang
Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan.
Faculty of Natural Sciences and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam.
Polymers (Basel). 2021 May 4;13(9):1483. doi: 10.3390/polym13091483.
The utilization of pectin-containing by-products may be useful in a variety of fields. This study aims to establish the processing of pectin-containing by-products to produce pectinases using TKU050 strain. In this study, several kinds of agricultural pectin-containing by-products from banana (banana peel), rice (rice bran), orange (orange peel), coffee (spent coffee grounds), and wheat (wheat bran) were utilized to provide carbon sources for the production of a pectinase by TKU050. TKU050 expressed the highest pectinase productivity (0.76 U/mL) on 0.5% wheat bran-containing medium at 37°C for four days. A 58 kDa pectinase was purified from the four-day cultured medium fermented under optimized culture conditions with 7.24% of a recovery ratio and 0.51 U/mg of specific activity, respectively. The optimum temperature, optimum pH, thermal stability, and pH stability of the TKU050 pectinase were 50 °C, pH 6, <50 °C, and pH 6-9, respectively. The TKU050 pectinase was inhibited by sodium dodecyl sulfate and Cu. The reducing sugar obtained by hydrolyzing banana peel with TKU050 pectinase showed the growth-enhancing effect on the growth of four tested lactic acid bacteria.
含果胶副产品的利用在多个领域可能会很有用。本研究旨在建立利用 TKU050 菌株处理含果胶副产品以生产果胶酶的方法。在本研究中，利用了几种来自香蕉（香蕉皮）、大米（米糠）、橙子（橙皮）、咖啡（咖啡渣）和小麦（麦麸）的农业含果胶副产品，为 TKU050 生产果胶酶提供碳源。在 37°C 下于含 0.5% 麦麸的培养基中培养四天，TKU050 表现出最高的果胶酶生产率（0.76 U/mL）。从在优化培养条件下发酵四天的培养基中纯化出一种 58 kDa 的果胶酶，回收率分别为 7.24%，比活性为 0.51 U/mg。TKU050 果胶酶的最适温度、最适 pH、热稳定性和 pH 稳定性分别为 50°C、pH 6、<50°C 和 pH 6 - 9。TKU050 果胶酶受到十二烷基硫酸钠和铜的抑制。用 TKU050 果胶酶水解香蕉皮得到的还原糖对四种受试乳酸菌的生长具有促进作用。
