Mavangira Vengai, Kuhn Matthew J, Abuelo Angel, Morisseau Christophe, Hammock Bruce D, Sordillo Lorraine M
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
Department of Entomology and Nematology, University of California Davis, Davis, CA 95616, USA.
Antioxidants (Basel). 2021 May 20;10(5):812. doi: 10.3390/antiox10050812.
Bovine coliform mastitis presents treatment challenges because of systemic inflammation and oxidative stress. Soluble epoxide hydrolase (sEH) is a promising therapeutic target in conditions characterized by inflammation and oxidative stress but has not been evaluated in cattle. We compared sEH activity and oxidant status in healthy Holstein dairy cows to those with systemic coliform mastitis ( = 5/group) using complementary approaches. First, the activity of sEH on [H]--diphenyl-propene oxide (tDPPO) was assessed ex vivo using tissue homogenates (mammary, liver, and kidney). Second, the concentrations of sEH substrates and metabolites in plasma, milk, and urine were determined as an index of in vivo sEH activity. Oxidant status was assessed in serum and milk. Data were analyzed by non-parametric methods. Metabolism of tDPPO was greater in mammary tissues from cows with coliform mastitis compared to controls. In contrast, ratios of sEH substrates and metabolites predicted lower sEH activity in cows with coliform mastitis than controls. Milk oxidant status showed greater prooxidant levels in coliform mastitis cows. Cows with coliform mastitis exhibit increased sEH activity in mammary tissue; at the same time, milk oxidant status is increased. Future studies should characterize sEH activity and oxidant status patterns and explore therapies targeting sEH during coliform mastitis.
由于全身性炎症和氧化应激，牛大肠埃希菌性乳腺炎带来了治疗挑战。可溶性环氧化物水解酶（sEH）是炎症和氧化应激相关病症中一个有前景的治疗靶点，但尚未在牛身上进行评估。我们采用互补方法，比较了健康荷斯坦奶牛与患有全身性大肠埃希菌性乳腺炎的奶牛（每组 = 5头）的sEH活性和氧化状态。首先，使用组织匀浆（乳腺、肝脏和肾脏）在体外评估sEH对[H] - -二苯基环氧丙烷（tDPPO）的活性。其次，测定血浆、牛奶和尿液中sEH底物和代谢物的浓度，作为体内sEH活性的指标。在血清和牛奶中评估氧化状态。数据采用非参数方法进行分析。与对照组相比，患有大肠埃希菌性乳腺炎的奶牛乳腺组织中tDPPO的代谢更高。相反，sEH底物和代谢物的比率表明，患有大肠埃希菌性乳腺炎奶牛的sEH活性低于对照组。牛奶氧化状态显示，大肠埃希菌性乳腺炎奶牛的促氧化剂水平更高。患有大肠埃希菌性乳腺炎的奶牛乳腺组织中sEH活性增加；同时，牛奶氧化状态增加。未来的研究应明确sEH活性和氧化状态模式，并探索在大肠埃希菌性乳腺炎期间针对sEH的治疗方法。
