García-Galán Ana, Seva Juan, Gómez-Martín Ángel, Ortega Joaquín, Rodríguez Francisco, García-Muñoz Ángel, De la Fe Christian
Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
Department of Anatomy and Comparative Pathological Anatomy, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
Animals (Basel). 2021 May 20;11(5):1470. doi: 10.3390/ani11051470.
Bovine respiratory disease (BRD) is an important viral and/or bacterial disease that mainly affects feedlot calves. The involvement of in BRD can lead to chronic pneumonia poorly responsive to antimicrobial treatment. Caseonecrotic bronchopneumonia is a pulmonary lesion typically associated with . In Spain, is widely distributed in the feedlots and circulating isolates are resistant to most antimicrobials in vitro. However, the role of this species in clinical respiratory disease of feedlot calves remains unknown. Furthermore, available data are relative to a fixed panel of antimicrobials commonly used to treat BRD, but not to the specific set of antimicrobials that have been used for treating each animal. This study examined 23 feedlot calves raised in southeast Spain (2016-2019) with clinical signs of respiratory disease unresponsive to treatment. The presence of was investigated through bacteriology (culture and subsequent PCR), histopathology and immunohistochemistry. The pathogen was found in 86.9% (20/23) of the calves, mainly in the lungs (78.26%; 18/23). Immunohistochemistry revealed antigens in 73.9% (17/23) of the calves in which caseonecrotic bronchopneumonia was the most frequent lesion (16/17). Minimum inhibitory concentration assays confirmed the resistance of a selection of 12 isolates to most of the antimicrobials specifically used for treating the animals in vivo. These results stress the importance of in the BRD affecting feedlot calves in Spain.
牛呼吸道疾病（BRD）是一种重要的病毒和/或细菌疾病，主要影响饲养场的犊牛。[未提及的某种病原体]参与BRD可导致对抗菌治疗反应不佳的慢性肺炎。干酪样坏死性支气管肺炎是一种通常与[未提及的某种病原体]相关的肺部病变。在西班牙，[未提及的某种病原体]在饲养场广泛分布，并且循环分离株在体外对大多数抗菌药物耐药。然而，该菌种在饲养场犊牛临床呼吸道疾病中的作用仍不清楚。此外，现有数据是关于通常用于治疗BRD的一组固定抗菌药物，而非用于治疗每只动物的特定抗菌药物组合。本研究检查了2016 - 2019年在西班牙东南部饲养的23头有呼吸道疾病临床症状且治疗无反应的饲养场犊牛。通过细菌学（培养及后续PCR）、组织病理学和免疫组织化学研究[未提及的某种病原体]的存在情况。在86.9%（20/23）的犊牛中发现了该病原体，主要在肺部（78.26%；18/23）。免疫组织化学在73.9%（17/23）的犊牛中检测到[未提及的某种病原体]抗原，其中干酪样坏死性支气管肺炎是最常见的病变（16/17）。最低抑菌浓度测定证实了从12株分离株中挑选的部分菌株对体内专门用于治疗这些动物的大多数抗菌药物耐药。这些结果强调了[未提及的某种病原体]在西班牙影响饲养场犊牛的BRD中的重要性。
