Rigotto Caruso Glaucia, Tonani Ludmilla, Marcato Priscyla Daniely, von Zeska Kress Marcia Regina
Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto 15040-903, SP, Brazil.
Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto 15040-903, SP, Brazil.
Antibiotics (Basel). 2021 May 12;10(5):569. doi: 10.3390/antibiotics10050569.
Antimicrobial photodynamic therapy (APDT) and silver nanoparticles (AgNPs) are known as promising alternatives for the control of microorganisms. This study aims to evaluate the antifungal activity of APDT, particularly by using the association of low concentrations of phenothiazinium photosensitizers (PS) methylene blue (MB), new methylene blue N (NMBN), and new methylene blue N Zinc (NMBN-Zn) in association with biosynthesized AgNPs. The AgNPs were characterized by UV-Vis spectrophotometry, transmission electron microscopy, and the dynamic light scattering method. The minimum inhibitory concentration of compounds in APDT against and was obtained and the Fractional Inhibitory Concentration Index determined the antifungal effect. The toxicity of compounds and associations in APDT were evaluated in . The AgNPs presented a surface plasmon band peak at 420 nm, hydrodynamic diameter of 86.72 nm, and zeta potential of -28.6 mV. AgNPs-PS showed a wider and displaced plasmon band peak due to PS ligands on the surface and decreased zeta potential. AgNPs-NMBN and AgNPs-NMBN-Zn associations presented synergistic effect in APDT with 15 J cm against both fungi and did not show toxicity to . Hence, the enhancement of antifungal activity with low concentrations of compounds and absence of toxicity makes APDT with AgNPs-PS a promising therapeutic alternative for fungal infections.
抗菌光动力疗法（APDT）和银纳米颗粒（AgNPs）被认为是控制微生物的有前景的替代方法。本研究旨在评估APDT的抗真菌活性，特别是通过使用低浓度吩噻嗪鎓类光敏剂（PS）亚甲蓝（MB）、新亚甲蓝N（NMBN）和新亚甲蓝N锌（NMBN-Zn）与生物合成的AgNPs联合使用。通过紫外可见分光光度法、透射电子显微镜和动态光散射法对AgNPs进行了表征。获得了APDT中化合物对[具体真菌名称1]和[具体真菌名称2]的最低抑菌浓度，并通过分数抑菌浓度指数确定了抗真菌效果。在[具体实验对象]中评估了APDT中化合物及其组合的毒性。AgNPs在420 nm处呈现表面等离子体带峰，流体动力学直径为86.72 nm，zeta电位为-28.6 mV。由于表面的PS配体，AgNPs-PS显示出更宽且位移的等离子体带峰，并且zeta电位降低。AgNPs-NMBN和AgNPs-NMBN-Zn组合在APDT中对两种真菌均表现出协同作用，光照剂量为15 J/cm²，且对[具体实验对象]未显示毒性。因此，低浓度化合物增强了抗真菌活性且无毒性，使得AgNPs-PS的APDT成为真菌感染的一种有前景的治疗选择。
