Bleackley Mark R, Samuel Monisha, Garcia-Ceron Donovan, McKenna James A, Lowe Rohan G T, Pathan Mohashin, Zhao Kening, Ang Ching-Seng, Mathivanan Suresh, Anderson Marilyn A
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia.
Bio21 Institute, University of Melbourne, Parkville, VIC, Australia.
Front Plant Sci. 2020 Jan 10;10:1610. doi: 10.3389/fpls.2019.01610. eCollection 2019.
Extracellular vesicles (EVs) represent a system for the coordinated secretion of a variety of molecular cargo including proteins, lipids, nucleic acids, and metabolites. They have an essential role in intercellular communication in multicellular organisms and have more recently been implicated in host-pathogen interactions. Study of the role for EVs in fungal biology has focused on pathogenic yeasts that are major pathogens in humans. In this study we have expanded the investigation of fungal EVs to plant pathogens, specifically the major cotton pathogen f. sp. . EVs isolated from f. sp. culture medium have a morphology and size distribution similar to EVs from yeasts such as and . A unique feature of the EVs from f. sp. is their purple color, which is predicted to arise from a napthoquinone pigment being packaged into the EVs. Proteomic analysis of f. sp. EVs revealed that they are enriched in proteins that function in synthesis of polyketides as well as proteases and proteins that function in basic cellular processes. Infiltration of f. sp. EVs into the leaves of cotton or plants led to a phytotoxic response. These observations lead to the hypothesis that f. sp. EVs are likely to play a crucial role in the infection process.
细胞外囊泡（EVs）是一种用于协调分泌多种分子货物（包括蛋白质、脂质、核酸和代谢物）的系统。它们在多细胞生物的细胞间通讯中起着至关重要的作用，最近还被认为参与宿主-病原体相互作用。对EVs在真菌生物学中的作用的研究主要集中在作为人类主要病原体的致病性酵母上。在本研究中，我们将对真菌EVs的研究扩展到植物病原体，特别是主要的棉花病原体 f. sp. 。从 f. sp. 培养基中分离出的EVs的形态和大小分布与来自酵母（如 和 ）的EVs相似。来自 f. sp. 的EVs的一个独特特征是它们的紫色，预计这是由于一种萘醌色素被包装到EVs中而产生的。对 f. sp. EVs的蛋白质组学分析表明，它们富含参与聚酮化合物合成的蛋白质以及蛋白酶和参与基本细胞过程的蛋白质。将 f. sp. EVs渗入棉花或 植物的叶片会导致植物毒性反应。这些观察结果提出了一个假设，即 f. sp. EVs可能在感染过程中起关键作用。
