Regecová Ivana, Výrostková Jana, Zigo František, Gregová Gabriela, Kováčová Mariana
Department of Food Hygiene, Technology and Safety, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Department of Animal Nutrition and Husbandry, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Antibiotics (Basel). 2021 May 12;10(5):570. doi: 10.3390/antibiotics10050570.
Antimicrobial and multidrug resistance is detected in nonaureus staphylococci, including , which commonly causes intramammary infections. Recent clinical studies point to the presence of methicillin-resistant Therefore, this study aims to determine the prevalence of this species in samples of sheep's milk and cheeses made from them. Isolates were identified by polymerase chain reaction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). A total of 208 staphylococcal isolates were identified. Of these, 18% were identified as . The antimicrobial resistance of the identified isolates was determined using the agar dilution method against penicillin, ceftaroline, teicoplanin, gentamicin, erythromycin, tetracycline, and ofloxacin. The highest resistance was found to penicillin (95%), tetracycline (86%), and oxacillin (81%). The highest sensitivity was confirmed for gentamicin (55%). The study also confirmed the presence of methicillin resistant staphylococcal isolates (30%) based on the phenotypic manifestation of antimicrobial resistance and detection of the presence of the gene. The study shows that the tested isolates (62%) were multidrug resistant. Resistance to two antibiotics was most often found (39%).
在非金黄色葡萄球菌中检测到了抗菌和多重耐药性，其中包括通常会引起乳房内感染的[具体菌种未给出]。近期的临床研究指出了耐甲氧西林[具体菌种未给出]的存在。因此，本研究旨在确定羊奶及其制成的奶酪样本中该菌种的流行情况。通过聚合酶链反应和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF）对分离株进行鉴定。总共鉴定出208株葡萄球菌分离株。其中，18%被鉴定为[具体菌种未给出]。使用琼脂稀释法针对青霉素、头孢托罗、替考拉宁、庆大霉素、红霉素、四环素和氧氟沙星测定已鉴定分离株的抗菌耐药性。发现对青霉素（95%）、四环素（86%）和苯唑西林（81%）的耐药性最高。确认对庆大霉素的敏感性最高（55%）。该研究还基于抗菌耐药性的表型表现和[具体基因未给出]基因存在的检测，证实了耐甲氧西林葡萄球菌分离株的存在（30%）。该研究表明，所测试的分离株（62%）具有多重耐药性。最常发现对两种抗生素耐药（39%）。
