Abulmeaty Mahmoud Mustafa Ali, Almajwal Ali Madi, Alnumair Khalid S, Razak Suhail, Hasan Mai Mohammed, Fawzy Amal, Farraj Abdullah Ibrahim, Abudawood Manal, Aljuraiban Ghadeer S
Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi Arabia.
Department of Medical Physiology, School of Medicine, Zagazig University, Zagazig 44519, Egypt.
Biology (Basel). 2021 May 7;10(5):413. doi: 10.3390/biology10050413.
Long-term continuous light exposure (CL) and western diet (WD) effects on Adropin expression, RORα, and Rev-erb-α nuclear receptors and energy homeostasis were studied in rats. Thirty-two male Wistar rats (250-290 g) were enrolled for 3 months in the following groups ( = 8/group): (a) Normal control group (NC), (b) CL group, (c) WD group, and (d) CL + WD group. Then, indirect calorimetry and food intake (FI) were measured. Finally, Adropin, hormone-sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL), and free fatty acids (FFA) were measured. Additionally, the histopathology and gene expression of Enho, RORα, and Rev-erb-α genes were done. The CL alone elevated the Adropin plasma level and gene expression, increased RORα expression, and decreased the Rev-erb-α nuclear receptor expression mainly in the liver and kidney. Besides, CL increased the total energy expenditure (TEE) and decreased the respiratory quotient. WD alone or in combination with the CL reversed gene expression of Enho, RORα, and Rev-erb-α. Combined CL and WD increased the TEE, reduced the food intake, increased the ATGL, and reduced the Adropin level in addition to widespread degenerative changes in the liver, spleen, and renal tissues. The deleterious effects of CL and WD on energy homeostasis may include Adropin with the involvement of the RORα and Rev-erb-α nuclear receptors.
研究了长期持续光照（CL）和西式饮食（WD）对大鼠内脂素表达、视黄酸受体相关孤儿受体α（RORα）和类维生素A受体α（Rev-erb-α）核受体以及能量稳态的影响。32只雄性Wistar大鼠（体重250 - 290克）被分为以下四组，每组8只，实验为期3个月：（a）正常对照组（NC），（b）持续光照组（CL），（c）西式饮食组（WD），（d）持续光照 + 西式饮食组（CL + WD）。然后，测量间接测热法和食物摄入量（FI）。最后，检测内脂素、激素敏感性脂肪酶（HSL）、脂肪细胞甘油三酯脂肪酶（ATGL）和游离脂肪酸（FFA）。此外，还进行了Enho、RORα和Rev-erb-α基因的组织病理学和基因表达检测。单独的持续光照主要在肝脏和肾脏中升高了内脂素血浆水平和基因表达水平，增加了RORα表达，并降低了Rev-erb-α核受体表达。此外，持续光照增加了总能量消耗（TEE）并降低了呼吸商。单独的西式饮食或与持续光照联合使用会使Enho、RORα和Rev-erb-α的基因表达发生逆转。持续光照与西式饮食联合增加了总能量消耗，减少了食物摄入量，增加了脂肪细胞甘油三酯脂肪酶，并降低了内脂素水平，此外还导致肝脏、脾脏和肾脏组织出现广泛的退行性变化。持续光照和西式饮食对能量稳态的有害影响可能包括内脂素以及视黄酸受体相关孤儿受体α和类维生素A受体α核受体的参与。
