Acar Ümit, Kesbiç Osman Sabri, Yılmaz Sevdan, İnanan Burak Evren, Zemheri-Navruz Fahriye, Terzi Funda, Fazio Francesco, Parrino Vincenzo
Bayramiç Vocational School, Çanakkale Onsekiz Mart University, Çanakkale 17100, Turkey.
Department of Microbiology, Veterinary Faculty, Kastamonu University, Kastamonu 37200, Turkey.
Animals (Basel). 2021 May 17;11(5):1431. doi: 10.3390/ani11051431.
The aim of this study was to detect effects of bitter orange () essential oil, commonly called neroli oil (NO) (0, 0.25, 0.50, 1, and 1.5% referred to as NO NO, NO, NO and NO, respectively) on growth performance output and expression levels of some growth-related genes in the muscle tissue and some immune-related genes in the head kidney and pathological differences in digestive system organs of common carp . The NO group had a large improvement in growth efficiency at the end of the 60-day feeding cycle. Real-time PCR (Bio RAD, USA) system was used to detect variations in gene expression levels. Furthermore, NO supplementation of up to 0.25% in muscle tissue controlled the release of growth hormone (GH) and insulin-like growth factor I (IGF-I). Furthermore, in the NO treatment category, immune response gene levels TNF-α, IL-8 and IL-1ß increased in head kidney tissue. In the histological examination of the liver and intestine, there were significant differences between fish fed with N and N diets. This study confirms that dietary supplementation of NO up to 0.25% can improve common carp growth efficiency and increase the expression of genes (GH and IGF-I) related to muscle growth, TNF-α, IL-8 and IL-1ß genes related to immune status, and liver and intestine histological status of common carp.
本研究的目的是检测苦橙（）精油（通常称为橙花油（NO），分别以0、0.25%、0.50%、1%和1.5%表示为NO₀、NO₀.₂₅、NO₀.₅₀、NO₁和NO₁.₅）对鲤鱼生长性能产出、肌肉组织中一些与生长相关基因以及头肾中一些与免疫相关基因的表达水平，以及消化系统器官病理差异的影响。在60天的投喂周期结束时，NO组的生长效率有了很大提高。使用实时荧光定量PCR（美国伯乐公司）系统检测基因表达水平的变化。此外，在肌肉组织中添加高达0.25%的NO可控制生长激素（GH）和胰岛素样生长因子I（IGF-I）的释放。此外，在NO处理组中，头肾组织中免疫反应基因TNF-α、IL-8和IL-1β的水平有所增加。在肝脏和肠道的组织学检查中，喂食N₀和N₀.₂₅日粮的鱼之间存在显著差异。本研究证实，日粮中添加高达0.25%的NO可提高鲤鱼的生长效率，并增加与肌肉生长相关的基因（GH和IGF-I）、与免疫状态相关的TNF-α、IL-8和IL-1β基因以及鲤鱼肝脏和肠道组织学状态的表达。
