Wongratanacheewin S, Charupatana C, Bunnag D, Sirisinha S
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1988 Mar;19(1):109-16.
The purpose of this study was to explore alternative method(s) to monitor the efficacy of anthelmintic treatment of patients with opisthorchiasis. Therefore, in our initial attempt, we studied the changes in antibody levels and lymphoproliferative responses in O. viverrini infected patients before and 2 months after successful praziquantel treatment. The results showed that although a substantial reduction of the antibody levels occurred after such a treatment, it did not occur in all patients. In those showing reduction, the final level were still above 2 standard deviations of the normal mean value. The reduction was more profound for IgG antibody. With regard to the IgA antibody isotype, the reduction was not as marked. In contrast, IgE antibody levels in most patients not only failed to decline, but instead, showed a tendency to be elevated after praziquantel treatment. Unlike the antibody levels, there was no alteration in the lymphoproliferative response to PHA stimulation and therefore this parameter is not useful for our intended objective. It was suggested that studies of a more specific O. viverrini component may be more reliable than the current method of parasitological examination of eggs in the feces of suspected individuals.
本研究的目的是探索监测华支睾吸虫病患者驱虫治疗效果的替代方法。因此，在我们的初步尝试中，我们研究了华支睾吸虫感染患者在成功接受吡喹酮治疗前和治疗后2个月抗体水平和淋巴细胞增殖反应的变化。结果显示，尽管治疗后抗体水平大幅下降，但并非所有患者均如此。在抗体水平下降的患者中，最终水平仍高于正常均值的2个标准差。IgG抗体的下降更为显著。对于IgA抗体同种型，下降并不明显。相比之下，大多数患者的IgE抗体水平不仅没有下降，反而在吡喹酮治疗后呈升高趋势。与抗体水平不同，对PHA刺激的淋巴细胞增殖反应没有变化，因此该参数对我们的预期目标没有用处。有人提出，研究更具特异性的华支睾吸虫成分可能比目前对疑似个体粪便中的虫卵进行寄生虫学检查的方法更可靠。
