Thammapalerd N, Tharavanij S, Nacapunchai D, Bunnag D, Radomyos P, Prasertsiriroj V
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1988 Mar;19(1):101-8.
Levels of antibody in sera of 78 patients with opisthorchiasis, 30 patients with other liver diseases, 10 patients with schistosomiasis and 30 healthy individuals were compared using three serodiagnostic tests, namely indirect haemagglutination (IHA), enzyme-linked immunosorbent assay (ELISA) and lectin immuno test (LIT). The geometric mean reciprocal titer in sera of opisthorchiasis patients was significantly higher than patients with other diseases, patients with schistosomiasis and healthy individuals (p less than 0.00001). After treatment with praziquantel, the antibody titers were decreased and became lowest 120 days after treatment. A statistically significant decrease from the pre-treatment sample was observed only at 120 days after infection and not earlier and only with ELISA (p = 0.03) and not with IHA and LIT (p greater than 0.05). Even with ELISA, significant decrease in antibody titer was apparent only when the pre-treatment sera had high enough antibody titer. ELISA was therefore better than the other two tests for the assessment of cure provided that the titer of pre-treatment sera was high.
采用间接血凝试验（IHA）、酶联免疫吸附测定（ELISA）和凝集素免疫试验（LIT）这三种血清学诊断试验，比较了78例华支睾吸虫病患者、30例其他肝病患者、10例血吸虫病患者和30名健康个体血清中的抗体水平。华支睾吸虫病患者血清中的几何平均倒数滴度显著高于其他疾病患者、血吸虫病患者和健康个体（p＜0.00001）。用吡喹酮治疗后，抗体滴度下降，治疗后120天降至最低。仅在感染后120天观察到与治疗前样本相比有统计学意义的下降，且出现时间不早，仅ELISA检测有下降（p = 0.03），IHA和LIT检测无下降（p＞0.05）。即使采用ELISA检测，只有当治疗前血清抗体滴度足够高时，抗体滴度才会出现显著下降。因此，如果治疗前血清滴度较高，ELISA在评估治愈情况方面优于其他两种检测方法。
