Wongratanacheewin S, Bunnag D, Vaeusorn N, Sirisinha S
Department of Microbiology, Faculty of Science, Hospital for Tropical Diseases, Bangkok, Thailand.
Am J Trop Med Hyg. 1988 Mar;38(2):356-62. doi: 10.4269/ajtmh.1988.38.356.
The humoral immune response in patients with opisthorchiasis was investigated using an enzyme-linked immunosorbent assay. IgG antibody reactive with Opisthorchis viverrini antigens was present in the serum of all patients. The infection also stimulated specific IgA and IgE antibody responses in most patients and, in practically all patients, there was a marked increase of total IgE. There was a moderate but significant correlation between serum IgG antibody level and severity of infection as judged from the quantity of eggs in the stool of the patients. There was also a significant elevation of antibody in the bile and serum of O. viverrini-infected patients who also had biliary obstruction. Analysis of paired samples from individual patients showed that while IgG was the predominant class of antibody in the serum of all patients, IgA was present at approximately the same level as IgG or higher in the bile of many patients. In addition to IgA and IgG antibodies, IgE antibody was also detectable in 50% of the bile samples. The high level of IgA antibody in the bile together with its presence in association with the secretory component suggested a selective transport and/or local production of IgA antibody by the hepatobiliary system of these patients.
采用酶联免疫吸附测定法对华支睾吸虫病患者的体液免疫反应进行了研究。所有患者血清中均存在与华支睾吸虫抗原反应的IgG抗体。该感染还在大多数患者中刺激了特异性IgA和IgE抗体反应，并且几乎在所有患者中，总IgE都有显著升高。根据患者粪便中虫卵数量判断，血清IgG抗体水平与感染严重程度之间存在中度但显著的相关性。在同时患有胆道梗阻的华支睾吸虫感染患者的胆汁和血清中，抗体也有显著升高。对个体患者的配对样本分析表明，虽然IgG是所有患者血清中主要的抗体类别，但在许多患者的胆汁中，IgA的水平与IgG大致相同或更高。除了IgA和IgG抗体外，50%的胆汁样本中也可检测到IgE抗体。胆汁中高水平的IgA抗体及其与分泌成分的结合表明，这些患者的肝胆系统对IgA抗体有选择性转运和/或局部产生。
