Akai P S, Pungpak S, Chaicumpa W, Kitikoon V, Ruangkunaporn Y, Bunnag D, Befus A D
Department of Microbiology and Infectious Diseases, University of Calgary, Canada.
Int J Parasitol. 1995 Aug;25(8):971-3. doi: 10.1016/0020-7519(94)00212-7.
We evaluated an enzyme-linked immunosorbent assay using crude parasite homogenates as a diagnostic test for Opisthorchis viverrini infection in humans. Serum antibody (Ab) responses to O. viverrini adult worm homogenate (AWH) and metacercaria homogenate (MH) were studied in 83 infected residents of an opisthorchiasis-endemic area in Thailand. Elevated levels of Ab persisted for over 1 year following curative treatment with praziquantel, and cross-reactivity to O. viverrini AWH and MH antigens was observed in sera from individuals with other parasitic infections. Serum Ab to crude AWH and MH are therefore unsuitable for immunodiagnosis since they may be non-specific and would not differentiate between ongoing and past infection.
我们评估了一种使用粗制寄生虫匀浆的酶联免疫吸附测定法，作为人类华支睾吸虫感染的诊断测试。在泰国华支睾吸虫病流行地区的83名受感染居民中，研究了血清抗体（Ab）对华支睾吸虫成虫匀浆（AWH）和后尾蚴匀浆（MH）的反应。用吡喹酮进行根治性治疗后，Ab水平升高持续超过1年，并且在患有其他寄生虫感染的个体血清中观察到对华支睾吸虫AWH和MH抗原的交叉反应。因此，针对粗制AWH和MH的血清Ab不适合用于免疫诊断，因为它们可能是非特异性的，并且无法区分正在进行的感染和既往感染。
