Tecnologico de Monterrey, School of Engineering and Sciences, Atizapan de Zaragoza 52926, Estado de Mexico, Mexico.
División de Ingeniería en Nanotecnología, Universidad Politécnica del Valle de México, Av. Mexiquense s/n esquina Av. Universidad Politécnica, Col. Villa Esmeralda, Tultitlan 54910, Estado de México, Mexico.
Molecules. 2021 May 13;26(10):2880. doi: 10.3390/molecules26102880.
The development of greener nano-constructs with noteworthy biological activity is of supreme interest, as a robust choice to minimize the extensive use of synthetic drugs. Essential oils (EOs) and their constituents offer medicinal potentialities because of their extensive biological activity, including the inhibition of fungi species. However, their application as natural antifungal agents are limited due to their volatility, low stability, and restricted administration routes. Nanotechnology is receiving particular attention to overcome the drawbacks of EOs such as volatility, degradation, and high sensitivity to environmental/external factors. For the aforementioned reasons, nanoencapsulation of bioactive compounds, for instance, EOs, facilitates protection and controlled-release attributes. Nanoliposomes are bilayer vesicles, at nanoscale, composed of phospholipids, and can encapsulate hydrophilic and hydrophobic compounds. Considering the above critiques, herein, we report the in-house fabrication and nano-size characterization of bioactive oregano essential oil ( L.) (OEO) molecules loaded with small unilamellar vesicles (SUV) nanoliposomes. The study was focused on three main points: (1) multi-compositional fabrication nanoliposomes using a thin film hydration-sonication method; (2) nano-size characterization using various analytical and imaging techniques; and (3) antifungal efficacy of as-developed OEO nanoliposomes against () by performing the mycelial growth inhibition test (MGI). The mean size of the nanoliposomes was around 77.46 ± 0.66 nm and 110.4 ± 0.98 nm, polydispersity index (PdI) of 0.413 ± 0.015, zeta potential values up to -36.94 ± 0.36 mV were obtained by dynamic light scattering (DLS). and spherical morphology was confirmed by scanning electron microscopy (SEM). The presence of OEO into nanoliposomes was displayed by attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. Entrapment efficiency values of 79.55 ± 6.9% were achieved for OEO nanoliposomes. In vitro antifungal activity of nanoliposomes tested against strains revealed that OEO nanoliposomes exhibited the highest MGI, 81.66 ± 0.86%, at a concentration of 1.5 µL/mL compared to the rest of the formulations. In summary, this work showed that bioactive OEO molecules with loaded nanoliposomes could be used as natural antifungal agents for therapeutical purposes against .
开发具有显著生物活性的更环保的纳米结构具有重要意义，因为这是一种强有力的选择，可以最大限度地减少合成药物的广泛使用。精油（EOs）及其成分因其广泛的生物活性而具有药用潜力，包括抑制真菌物种。然而，由于其挥发性、低稳定性和受限的给药途径，它们作为天然抗真菌剂的应用受到限制。纳米技术受到特别关注，以克服 EOs 的缺点，如挥发性、降解和对环境/外部因素的高敏感性。出于上述原因，例如，将生物活性化合物（如 EOs）包封在纳米胶囊中，可以实现保护和控制释放特性。纳米脂质体是由磷脂组成的纳米级双层囊泡，可以包封亲水性和疏水性化合物。考虑到上述批评，在这里，我们报告了使用薄膜水化-超声法制备和纳米尺寸表征负载有小单层囊泡（SUV）纳米脂质体的生物活性牛至精油（ L.）（OEO）分子。该研究集中在三个主要方面：（1）使用薄膜水化-超声法制备多成分纳米脂质体；（2）使用各种分析和成像技术进行纳米尺寸表征；（3）通过进行菌丝生长抑制试验（MGI），研究开发的 OEO 纳米脂质体对（）的抗真菌功效。纳米脂质体的平均粒径约为 77.46 ± 0.66nm，多分散指数（PdI）为 0.413 ± 0.015，zeta 电位值高达-36.94 ± 0.36mV，通过动态光散射（DLS）获得。扫描电子显微镜（SEM）证实了球形形态。ATR-FTIR 光谱显示 OEO 存在于纳米脂质体中。OEO 纳米脂质体的包封效率值为 79.55 ± 6.9%。体外抗真菌活性试验表明，OEO 纳米脂质体对菌株的 MGI 最高，为 81.66 ± 0.86%，在 1.5μL/mL 浓度下，与其他制剂相比。总之，这项工作表明，负载有纳米脂质体的生物活性 OEO 分子可用作治疗性天然抗真菌剂，用于对抗 。
