School of Engineering and Sciences, Tecnologico de Monterrey, Atizapan de Zaragoza 52926, Estado de Mexico, Mexico.
Institute of Advanced Materials for Sustainable Manufacturing, Tecnologico de Monterrey, Monterrey 64849, Nuevo León, Mexico.
Molecules. 2022 Sep 5;27(17):5728. doi: 10.3390/molecules27175728.
Nanoliposomes, bilayer vesicles at the nanoscale, are becoming popular because of their safety, patient compliance, high entrapment efficiency, and prompt action. Several notable biological activities of natural essential oils (EOs), including fungal inhibition, are of supreme interest. As developed, multi-compositional nanoliposomes loaded with various concentrations of clove essential oil (CEO) and tea tree oil (TTO) were thoroughly characterized to gain insight into their nano-size distribution. The present work also aimed to reconnoiter the sustainable synthesis conditions to estimate the efficacy of EOs in bulk and EO-loaded nanoliposomes with multi-functional entities. Following a detailed nano-size characterization of in-house fabricated EO-loaded nanoliposomes, the antifungal efficacy was tested by executing the mycelial growth inhibition (MGI) test using fungi as a test model. The dynamic light scattering (DLS) profile of as-fabricated EO-loaded nanoliposomes revealed the mean size, polydispersity index (PdI), and zeta potential values as 37.12 ± 1.23 nm, 0.377 ± 0.007, and -36.94 ± 0.36 mV, respectively. The sphere-shaped morphology of CEO and TTO-loaded nanoliposomes was confirmed by a scanning electron microscope (SEM). The existence of characteristic functional bands in all tested counterparts was demonstrated by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy. Compared to TTO-loaded nanoliposomes, the CEO-loaded nanoliposomes exhibited a maximum entrapment efficacy of 91.57 ± 2.5%. The CEO-loaded nanoliposome fraction, prepared using 1.5 µL/mL concentration, showed the highest MGI of 98.4 ± 0.87% tested against strains compared to the rest of the formulations.
纳米脂质体是纳米尺度的双层囊泡，由于其安全性、患者顺应性、高包封效率和快速作用而受到关注。天然精油（EOs）具有多种显著的生物学活性，包括抗真菌作用，这引起了人们的极大兴趣。研究人员开发了负载不同浓度丁香精油（CEO）和茶树油（TTO）的多成分纳米脂质体，对其纳米尺寸分布进行了全面表征。本工作还旨在探索可持续的合成条件，以评估精油的整体功效和负载精油的纳米脂质体的多效实体功效。在对自制的负载精油的纳米脂质体进行详细的纳米尺寸表征后，通过使用真菌作为测试模型执行菌丝生长抑制（MGI）测试来测试其抗真菌功效。所制备的负载精油的纳米脂质体的动态光散射（DLS）图谱显示，平均粒径、多分散指数（PdI）和zeta 电位值分别为 37.12 ± 1.23nm、0.377 ± 0.007 和 -36.94 ± 0.36mV。扫描电子显微镜（SEM）证实了 CEO 和 TTO 负载的纳米脂质体的球形形态。衰减全反射傅里叶变换红外（ATR-FTIR）光谱证明了所有测试对照物中存在特征功能带。与 TTO 负载的纳米脂质体相比，CEO 负载的纳米脂质体的包封效率最高，为 91.57 ± 2.5%。与其他配方相比，用 1.5µL/mL 浓度制备的 CEO 负载纳米脂质体部分对测试的菌株表现出最高的 MGI，为 98.4 ± 0.87%。
