Recombinant Expression of Aflatoxin B-Degrading Enzyme (TV-AFBD) in Engineering GS115 and Application in AFB Degradation in AFB-Contaminated Peanuts.

Aflatoxins seriously threaten the health of humans and animals due to their potential carcinogenic properties. Enzymatic degradation approach is an effective and environmentally friendly alternative that involves changing the structure of aflatoxins. In this study, aflatoxin B-degrading enzyme gene (TV-AFBD) was integrated into the genome of GS115 by homologous recombination approach. The recombinant TV-AFBD was expressed in engineering with a size of approximately 77 kDa under the induction of methanol. The maximum activity of TV-AFBD reached 17.5 U/mL after the induction of 0.8% ethanol (/) for 84 h at 28 °C. The AFB proportion of 75.9% was degraded using AFB standard sample after catalysis for 12 h. In addition, the AFB proportion was 48.5% using AFB-contaminated peanuts after the catalysis for 18 h at 34 °C. The recombinant TV-AFBD would have good practical application value in AFB degradation in food crops. This study provides an alternative degrading enzyme for the degradation of AFB in aflatoxin-contaminated grain and feed via enzymatic degradation approach.