Identification of the Multiresistance Gene in Oxazolidinone-Susceptible and of Pig and Feed Origins.

Previous studies on the prevalence and transmission mechanism of oxazolidinone resistance gene in CoNS are lacking, which this study addresses. By screening 763 CoNS isolates from different sources of several livestock farms in Guangdong, China, 2018-2020, we identified that the was present in seven CoNS isolates of pig and feed origins. Species identification and multilocus sequence typing (MLST) confirmed that seven -positive CoNS isolates were composed of five ST64- and two isolates. All -positive isolates shared similar pulsed-field gel electrophoresis (PFGE) patterns. Transformation assays demonstrated all -positive isolates were able to transfer gene to RN4220. S1-PFGE and whole-genome sequencing (WGS) revealed the presence of -carrying plasmids in size around 54.7 kb. The plasmid pY80 was 55,758 bp in size and harbored the heavy metal resistance gene and antimicrobial resistance genes, , , and (L). The regions (IS--IS) in plasmid pY80 were identified in spp. and spp. with different genetic and source backgrounds. In conclusion, this was the first report about the gene in and , and IS may play an important role in the dissemination of among different Gram-positive bacteria.