Huang Jinhu, Li Aijuan, Wang Mengli, Gu Shushu, Hu He, Wang Xiaoming, Wang Liping
MOE Joint International Research Laboratory of Animal Health and Food Safety, Risk Assessment Center of Veterinary Drug Residue and Antimicrobial Resistance, Center for Veterinary Drug Research and Evaluation, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Nanjing Animal Husbandry and Veterinary Station, Nanjing 210012, China.
Transbound Emerg Dis. 2025 Jul 13;2025:9275403. doi: 10.1155/tbed/9275403. eCollection 2025.
Linezolid and penicillin are critical for treating multidrug resistant (MDR) Gram-positive infections, but the emergence of resistance to both seriously threatens public health. Here, we first report the cocarrying (oxazolidinone resistance) and (β-lactam resistance) genes by the plasmid in a strain of HDC14-2 derived from porcine. The isolate also exhibits MDR phenotypes to phenicols, oxazolidinones, tetracyclines, β-lactams, aminoglycosides, macrolides, and lincosamides. Whole-genome sequencing (WGS) revealed these resistance genes, along with (), (), , (B), ()-(), , , (E), (B), , and , were clustered in a novel MDR region flanked by IS elements on plasmid pHDC14-2.133K. This IS-bounded MDR region formed translocatable units (TUs), including an IS- TU that was also identified on a secondary plasmid, pHDC14-2.27K. Functional assays demonstrated the excisability and mobility of these TUs, indicating its potential ability integration into other plasmids or chromosomes. Critically, electrotransformation confirmed the transfer of pHDC14-2.27K (-carrying) to JH2-2, with retained TU activity and minimal fitness cost. This study provides the evidence of colocalized and on plasmids in enterococci, highlighting their role in disseminating pan-resistance among bacteria. Although is not an important pathogenic bacterium to humans and animals, but its potential risk to horizontally spread of these resistance genes important in medicine still cannot be ignored.
利奈唑胺和青霉素对于治疗多重耐药（MDR）革兰氏阳性菌感染至关重要，但对这两种药物的耐药性出现严重威胁着公众健康。在此，我们首次报道了源自猪的屎肠球菌HDC14 - 2菌株中由质粒共携带（恶唑烷酮抗性）和（β - 内酰胺抗性）基因。该分离株还对酚类、恶唑烷酮类、四环素类、β - 内酰胺类、氨基糖苷类、大环内酯类和林可酰胺类表现出多重耐药表型。全基因组测序（WGS）揭示这些耐药基因，连同（）、（）、 、（B）、（） - （）、 、 、（E）、（B）、 、 和 ，聚集在质粒pHDC14 - 2.133K上由IS元件侧翼包围的一个新的多重耐药区域。这个由IS界定的多重耐药区域形成了可转移单元（TUs），包括一个在第二个质粒pHDC14 - 2.27K上也被鉴定出的IS - TU。功能分析证明了这些TUs的可切除性和移动性，表明其整合到其他质粒或染色体中的潜在能力。至关重要的是，电转化证实了携带 - 的pHDC14 - 2.27K转移到粪肠球菌JH2 - 2，保留了TU活性且适应性代价最小。本研究提供了屎肠球菌中质粒上 和 共定位的证据，突出了它们在细菌间传播泛耐药性中的作用。虽然屎肠球菌对人和动物不是重要的病原菌，但其对医学上这些重要耐药基因水平传播的潜在风险仍不能忽视。
