Yano Satoshi, Wang Jinyun, Kabayama Shigeru, Hara Taichi
Laboratory of Food and Life Science, Faculty of Human Sciences, Waseda University, Tokorozawa 359-1192, Japan.
Nihon Trim Co. Ltd., Oyodonaka, Kita-ku, Osaka 531-0076, Japan.
Antioxidants (Basel). 2021 May 19;10(5):801. doi: 10.3390/antiox10050801.
Excessive alcohol consumption can cause multi-systemic diseases. Among them, alcoholic liver disease is the most frequent and serious disease. Electrolytic hydrogen water (EHW) is produced at the cathode during electrolysis of water and contains a large amount of molecular hydrogen and a low content of platinum nanoparticles with alkaline properties. In this study, we found that EHW inhibits ethanol-induced cytotoxicity by decreasing the intracellular acetaldehyde, a toxic substance produced by ethanol degradation, in hepatocyte cell lines HepG2. Analysis of the mechanism of action revealed that EHW inhibits the metabolism of ethanol to acetaldehyde by suppressing alcohol dehydrogenase. EHW also promotes the metabolism of acetaldehyde to acetic acid by activating aldehyde dehydrogenase, which plays to reduce aldehyde toxicity and intracellular reactive oxygen species in HepG2 cells. These functions were correlated with the concentration of molecular hydrogen in EHW, and were abolished by degassing treatment, suggesting that molecular hydrogen may contribute as a functional factor in the suppression of ethanol-induced hepatocellular damage. Furthermore, hydrogen water with high dissolved hydrogen molecule showed the same hepatocellular protective effect against ethanol as the EHW. These results suggest that EHW may be useful in the prevention of alcoholic liver disease.
过量饮酒会导致多系统疾病。其中，酒精性肝病是最常见且最严重的疾病。电解水制得的电解氢水（EHW）在电解过程中于阴极产生，含有大量分子氢以及少量具有碱性的铂纳米颗粒。在本研究中，我们发现EHW通过降低肝细胞系HepG2中乙醇降解产生的有毒物质细胞内乙醛水平，抑制乙醇诱导的细胞毒性。作用机制分析表明，EHW通过抑制乙醇脱氢酶来抑制乙醇代谢为乙醛。EHW还通过激活乙醛脱氢酶促进乙醛代谢为乙酸，这起到降低HepG2细胞中乙醛毒性和细胞内活性氧的作用。这些功能与EHW中分子氢的浓度相关，并通过脱气处理消除，表明分子氢可能作为抑制乙醇诱导的肝细胞损伤的功能因子发挥作用。此外，具有高溶解氢分子的氢水对乙醇显示出与EHW相同的肝细胞保护作用。这些结果表明EHW可能对预防酒精性肝病有用。
